Evaluation of anti-bacterial and anti-oxidant potential of andrographolide and echiodinin isolated from callus culture of Andrographis paniculata Nees
Evaluation of anti-bacterial and anti-oxidant potential of andrographolide and echiodinin isolated from callus culture of Andrographis paniculata Nees
复制标题
DOI：
10.1016/s2221-1691(13)60123-9
复制
发表时间：
2013-08-01
影响因子：
1.7
通讯作者：
Gopal, Ghanta Rama
中科院分区：
文献类型：
--
作者：
Arifullah, Mohmmed;Namsa, Nima Dandu;Gopal, Ghanta Rama
目的：目的：评价穿心莲中穿心莲内酯（andrographitaxel，AND）和Echiodinin（ECH）的体外抗菌活性和抗氧化活性。方法：采用微量肉汤稀释法和DPPH法分别对分离得到的AND和ECH进行体外抗菌活性和抗氧化活性的研究。采用电喷雾质谱、核磁共振等方法进行结构鉴定（H-1和C-13）和IR光谱。AND对大多数测试菌株（包括耻垢分枝杆菌）有效，显示广谱生长抑制活性，对金黄色葡萄球菌具有最小抑制浓度值（100 μ g/mL），嗜热链球菌（350 μ g/mL）枯草芽孢杆菌（100 μ g/mL），大肠埃希菌（50 μ g/mL）、耻垢分枝杆菌（200 μ g/mL）、肺炎克雷伯氏菌（100 μ g/mL）和铜绿假单胞菌（200 μ g/mL）。ECH在浓度高于225 μ g/mL时对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌和铜绿假单胞菌表现出特异性抗菌活性。AND和ECH对白色念珠菌和酵母菌均无抑制作用。结论：AND和ECH具有良好的抗菌活性和一定的自由基清除活性，为AND和ECH的民间药用提供了科学依据。
Objective: To evaluate the anti-bacterial and anti-oxidant activity of andrographolide (AND) and echiodinin (ECH) of Andrographis paniculata.Methods: In this study, an attempt has been made to demonstrate the anti-microbial and antioxidant activity of isolated AND and ECH by broth micro-dilution method and 2,2-diphenyl-2-picryl-hydrazyl (DPPH) assay, respectively. Structure elucidation was determined by electro-spray ionization-MSD, NMR (H-1 and C-13) and IR spectra.Results: AND was effective against most of the strains tested including Mycobacterium smegmatis, showing broad spectrum of growth inhibition activity with Minimum inhibitory concentration values against Staphylococcus aureus (100 mu g/mL), Streptococcus thermophilus (350 mu g/mL) Bacillus subtilis (100 mu g/mL), Escherichia coli (50 mu g/mL), Mycobacterium smegmatis (200 mu g/mL), Klebsiella pneumonia (100 mu g/mL), and Pseudomonas aeruginosa (200 mu g/mL). ECH showed specific anti-bacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa at a concentration higher than 225 mu g/mL. Both AND and ECH were not effective against the two yeast strains, Candida albicans and Saccharomyces cereal-sine tested in this study.Conclusion: This preliminary study showed promising anti-bacterial activity and moderate free radical scavenging activity of AND and ECH, and it may provide the scientific rationale for its popular folklore medicines.