Morphology and Composition of the Spider Major Ampullate Gland and Dragline Silk
Morphology and Composition of the Spider Major Ampullate Gland and Dragline Silk
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DOI：
10.1021/bm400898t
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发表时间：
2013-08-01
影响因子：
6.2
通讯作者：
Rising, Anna
中科院分区：
文献类型：
--
作者：
Andersson, Marlene;Holm, Lena;Rising, Anna
蜘蛛丝由独特的蛋白质--蛛丝--制成，作为蛋白质溶液（涂料）分泌并储存在专门的腺体中。大壶腹腺是拖丝的来源，由尾部、囊和细长的导管组成。对于这个腺体，已经描述了几种不同类型的上皮细胞和颗粒，但很大程度上不知道它们如何与蛛丝蛋白产生相关。也没有解决大蛛丝的哪些部分最终形成最终的丝，并且已经提出缺乏N-末端结构域（NT）。在这里，我们表明，NT是存在于涂料和整个拖动，线丝纤维，包括皮肤层，和主要壶状尾和囊由三个不同的和明确划分的区域（A-C），每个具有不同的上皮细胞类型。最后，我们发现，蛛丝蛋白产生于A和B区上皮细胞，而C区颗粒缺乏蛛丝蛋白。
Spider silk is made of unique proteins-spidroins-secreted and stored as a protein solution (dope) in specialized glands. The major ampullate gland, source of the dragline silk, is composed of a tail, a sac and an elongated duct. For this gland, several different types of epithelial cells and granules have been described, but it is largely unknown how they correlate with spidroin production. It is also not settled what parts of the large spidroins end up in the final silk, and it has been suggested that the N-terminal domain (NT) is lacking. Here we show that NT is present in the dope and throughout drag,line silk fibers, including the skin layer, and that the major ampullate tail and sac consist of three different and sharply demarcated zones (A-C), each with a distinct epithelial cell type. Finally, we show that spidroins are produced in the A and B zone epithelia, while the C zone granules lack spidroins.