Harvesting the maximum length of sciatic nerve from adult mice: a step-by-step approach.
Harvesting the maximum length of sciatic nerve from adult mice: a step-by-step approach.
复制标题
DOI：
10.1186/1756-0500-7-714
复制
发表时间：
2014-10-10
影响因子：
1.8
通讯作者：
Cheah PS
中科院分区：
文献类型：
--
作者：
Bala U;Tan KL;Ling KH;Cheah PS
在过去的几十年里，已经进行了许多关于周围神经损伤或再生的研究。小鼠已被广泛用于许多这些研究，坐骨神经是最有针对性和首选的神经。因此，用于收获足以用于不同分析的最大长度的小鼠坐骨神经的技术将是非常有价值的。在这里，我们描述了一个简单的一步一步的指导收获的最大长度的小鼠坐骨神经，并比较收获的神经的长度收集与建议的方法与使用传统的大腿中部切口方法获得的神经。暴露坐骨神经，同时用一只手将两个后肢握在一起，并将尾部向相反方向轻轻拉开。通过解剖远端和近端的神经来追踪神经，并小心地收获。使用所提出的获取方法和大腿中部切口方法获得的坐骨神经的总平均长度分别为22.60 ± 1.62 mm和7.0 ± 0.76 mm。这种长度的收获神经允许进一步解剖成几个节段，可用于额外的独立分析，如组织化学/组织学分析和RNA或蛋白质提取。这里描述的方法与大腿中部切口方法相比具有几个优点，因为它：i）允许获取最大长度的坐骨神经，ii）允许同时获取两条坐骨神经，iii）节省时间; iv）不需要广泛的兽医解剖学知识;以及v）提供无障碍解剖。
Over the past several decades, many studies concerning peripheral nerve damage or regeneration have been performed. Mice have been widely used for many of these studies, with the sciatic nerve being the most targeted and preferred nerve. Therefore, techniques for harvesting mouse sciatic nerves of a maximum length that is sufficient for different analyses will be highly valuable. Here we describe a simple step-by-step guide for harvesting the maximum length of mouse sciatic nerve and compare the length of the harvested nerves gathered with the proposed method with nerves obtained using a conventional mid-thigh incision approach. The sciatic nerve was exposed while holding both hind limbs together in one hand and the tail was gently pulled away in the opposite direction. The nerve was traced by dissecting through its course both distally and proximally and was carefully harvested. The total average length of the sciatic nerves obtained using the proposed harvesting method and the mid-thigh incision method was 22.60 ± 1.62 mm and 7.0 ± 0.76 mm, respectively. This length of harvested nerve allows further dissection into several segments that can be used for additional independent analyses such as histochemical/histological analysis and RNA or protein extraction. The approach described here has several advantages over mid-thigh incision methods in that it: i) allows harvesting of maximum lengths of the sciatic nerve ii) allows simultaneous harvesting of both sciatic nerves, iii) provides time savings; iv) requires no extensive knowledge of veterinary anatomy; and v) provides hassle-free dissection.