Short-term continuous and pulse Pb exposure causes negative effects on skin histomorphological structure and bacterial composition of adult Pelophylax nigromaculatus
Short-term continuous and pulse Pb exposure causes negative effects on skin histomorphological structure and bacterial composition of adult Pelophylax nigromaculatus
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DOI：
10.1007/s11356-022-19743-5
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发表时间：
2022-03
影响因子：
5.8
通讯作者：
Yang Liu;Min-yi Huang;Yujiao Wang;Ren-yan Duan;Junling Guo;Xiaohong Cao;Xiang Xu
中科院分区：
文献类型：
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作者：
Yang Liu;Min-yi Huang;Yujiao Wang;Ren-yan Duan;Junling Guo;Xiaohong Cao;Xiang Xu
铅 (Pb) 是一种有毒重金属，通常以水中的脉冲形式存在于环境中。传统的毒性测试通常在连续浓度条件下进行，没有考虑脉冲暴露对水生生物的影响。本研究旨在评价短期连续和脉冲Pb暴露对黑斑斑鳢皮肤细菌和组织形态结构的影响。结果显示，与对照组（CON）和Pb连续暴露组（CEPb）相比，Pb脉冲暴露组（PEPb）呈现出最小尺寸的颗粒腺体，这会干扰皮肤的通透性和分泌功能，使个体对外部污染更加敏感。铅暴露显着改变了皮肤细菌的组成和多样性。与 CON 和 CEPb 组相比，PEPb 组显示有害细菌（例如拟杆菌和金杆菌）丰度显着增加，而有益细菌（例如假单胞菌）丰度下降。 PICRUSt软件显示，三组（CON、CEPb和PEPb）皮肤细菌的代谢途径存在差异。总体而言，本研究表明Pb脉冲暴露会加剧Pb对青蛙皮肤的毒性，为模拟青蛙健康背景下的短期重金属暴露提供了新的框架。 图解摘要
Lead (Pb) is a toxic heavy metal often present in the environment as a pulse in water. Traditional toxicity tests are usually carried out under conditions of continuous concentration, without considering the impact of pulse exposure on aquatic organisms. This study aimed to evaluate the effects of short-term continuous and pulse Pb exposures on the skin bacteria and histomorphological structure ofPelophylax nigromaculatus. Results showed that compared to the control (CON) and Pb continuous exposure group (CEPb), the Pb pulse exposure group (PEPb) showed the smallest size of granular glands, which would interfere with the permeability and secretory function of skin, making the individual more sensitive to external pollution. Lead exposure significantly changed the composition and diversity of skin bacteria. Compared to the CON and CEPb groups, the PEPb group showed a significant increase in the abundance of harmful bacteria (e.g.,BacteroidetesandChryseobacterium) and a decrease in the abundance of beneficial bacteria (e.g.,Pseudomonas). PICRUSt software showed that there were differences in the metabolic pathway of skin bacteria among the three groups (CON, CEPb, and PEPb). Overall, this study indicates that Pb pulse exposure can aggravate the toxicity of Pb for frog skin, providing a new framework for simulating short-term heavy metal exposure in the context of frog health.Graphical abstract