Alternative protein secretion:: The Mam1 ABC transporter supports secretion of M-factor linked GFP in fission yeast
Alternative protein secretion:: The Mam1 ABC transporter supports secretion of M-factor linked GFP in fission yeast
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DOI：
10.1016/j.bbrc.2005.10.156
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发表时间：
2005-12-30
影响因子：
3.1
通讯作者：
Jensen, MR
中科院分区：
文献类型：
--
作者：
Kjærulff, S;Müller, S;Jensen, MR
为了检查裂殖酵母 Maml ABC 转运蛋白是否可用于分泌异源蛋白质，从而绕过经典的分泌途径，我们分析了 M 因子前体的嵌合形式。结果表明，当与 mfin1 的氨基末端原序列和 CaaX 基序融合时，GFP 可以输出。这种分泌依赖于 Maml 转运蛋白，而不是经典的分泌途径。然而，GFP 的分泌效率相对较低，并且大部分报告蛋白被捕获在液泡膜中。我们的研究结果表明，Main1 ABC 蛋白是一种混杂的肽转运蛋白，可以容纳相对较大尺寸的球状蛋白。此外，我们的结果有助于定义天然 M 因子加工和分泌所需的序列。 (c) 2005 Elsevier Inc. 保留所有权利。
To examine whether the fission yeast Maml ABC transporter can be used for secretion of heterologous proteins, thereby bypassing the classical secretion pathway, we have analyzed chimeric forms of the M-factor precursor. It was demonstrated that GFP can be exported when fused to both the amino-terminal prosequence from mfin1 and a CaaX motif. This secretion was dependent on the Maml transporter and not the classical secretion pathway. The secretion efficiency of GFP, however, was relatively low and most of the reporter protein was trapped in the vacuolar membranes. Our findings suggest that the Main1 ABC protein is a promiscuous peptide transporter that can accommodate globular proteins of a relatively large size. Furthermore, our results help in defining the sequences required for processing and secretion of natural M-factor. (c) 2005 Elsevier Inc. All rights reserved.