Studying the penetration of fatty acids into human skin by ex vivo TOF-SIMS imaging
Studying the penetration of fatty acids into human skin by ex vivo TOF-SIMS imaging
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DOI：
10.1186/1559-4106-8-3
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发表时间：
2013-02-06
期刊：
影响因子：
2.1
通讯作者：
Briedis, Vitalis
中科院分区：
文献类型：
--
作者：
Kezutyte, Toma;Desbenoit, Nicolas;Briedis, Vitalis
脂肪酸类化学渗透促进剂（CPE）可引起角质层（SC）脂质基质的流化和扰动。通过飞行时间二次离子质谱（TOF-SIMS）成像研究了油酸、亚油酸、月桂酸和癸酸向人皮肤的渗透，并与脂肪酸对亲脂性模型药物托萘酯向人表皮和真皮的离体渗透的增强作用相关。使用Bronaugh型流通扩散池评价脂肪酸对托萘酯渗透到人皮肤中的增强作用。在体外渗透研究后，使用TOF-SIMS进行脂肪酸分子在人皮肤中的可视化和空间定位。通过离子图像和强度曲线，将油酸、亚油酸、月桂酸和癸酸渗透到人皮肤中与对照皮肤切片进行比较。只有油酸显著（P
Fatty acids classified as chemical penetration enhancers (CPEs) might cause the fluidization and perturbation of stratum corneum (SC) lipid matrix. The penetration of oleic, linoleic, lauric and capric acids into human skin was studied by time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging and related to fatty acids enhancing effect on lipophilic model drug tolnaftate penetration into human epidermis and dermis ex vivo. Fatty acid enhancing effect on tolnaftate penetration into human skin was evaluated using Bronaugh-type flow-through diffusion cells. After in vitro penetration studies visualization and spatial localization of fatty acid molecules in human skin were performed using TOF-SIMS. Penetration of oleic, linoleic, lauric and capric acids into human skin was compared to the control skin sections by ion images and intensity profiles. Only oleic acid significantly (P