FACTORS MODULATING THE EFFECT OF RETINOIDS ON CULTURED RETINAL-PIGMENT EPITHELIAL-CELL PROLIFERATION
FACTORS MODULATING THE EFFECT OF RETINOIDS ON CULTURED RETINAL-PIGMENT EPITHELIAL-CELL PROLIFERATION
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DOI：
10.3109/02713689209000749
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发表时间：
1992-08-01
影响因子：
2
通讯作者：
BUZNEY, SM
中科院分区：
文献类型：
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作者：
DOYLE, JW;DOWGIERT, RK;BUZNEY, SM
研究了几种天然维甲酸和13-顺式维甲酸对培养的牛视网膜色素上皮细胞增殖的影响。没有一个类维生素A测试是有毒的文化和所有，除了retinylpalmitate，抑制细胞增殖时，给予超过3天。类维生素A的相对效价为全反式维甲酸> 13-顺式维甲酸>全反式视黄醇与全反式视黄醇醛的混合物。培养的RPE细胞对视黄酸的摄取比对视黄醇的摄取少10倍。虽然维甲酸处理的文化表现出强烈的密度依赖性生长抑制，细胞增殖抑制稀疏文化比密集的。视黄酸对第一代牛、兔RPE细胞的增殖无明显抑制作用，但对第一代人RPE细胞的增殖有部分抑制作用。所有这些文化的敏感性，以生长抑制视黄酸增加，在随后的继代培养，但有没有影响的传代次数对视黄酸的摄取。这项研究表明，视网膜色素上皮细胞的增殖可以抑制视黄酸，但这些细胞的敏感性视黄酸的影响调制的孵育时间，体外老化，和细胞密度。
The effect of several naturally-occurring retinoids and 13-cis-retinoic acid on the proliferation of cultured bovine retinal pigment epithelial (RPE) cells was investigated. None of the retinoids tested were toxic to the cultures and all, except retinylpalmitate, inhibited cell proliferation when given for more than 3 days. The relative potencies of the retinoids were; all-trans-retinoic acid > 13-cis-retinoic acid > all-trans-retinol congruent-to all-trans-retinaldehyde. Uptake of retinoic acid by cultured RPE cells was 10-fold less than the uptake of retinol. Although retinoic acid-treated cultures showed strong density-dependent growth inhibition, cellular proliferation was inhibited more in sparse cultures than in dense ones. Retinoic acid did not significantly inhibit the proliferation of first passage bovine or rabbit RPE cells, but partially inhibited the proliferation of first passage human RPE cells. The sensitivity of all these cultures to growth inhibition by retinoic acid increased in subsequent subcultures, yet there was no effect of passage number on retinoic acid uptake. This study demonstrates that RPE cell proliferation can be inhibited by retinoic acid but the sensitivity of these cells to the retinoid's effects are modulated by incubation time, in vitro aging, and cell density.