Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases EcoRI, BglII and BamHI.
Analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases EcoRI, BglII and BamHI.
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马疱疹病毒 1 型基因组分析：限制性内切酶 EcoRI、BglII 和 BamHI 切割位点的排列。
DOI：
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发表时间：
1981
影响因子：
3.8
通讯作者：
Andrew J. Davison
中科院分区：
文献类型：
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作者：
Whalley Jm;Robertson Gr;Andrew J. Davison
使用限制性内切酶EcoRI、BglII和BamHI分析了澳大利亚马疱疹病毒1型（马流产病毒）的基因组，并构建了物理图谱。末端片段通过核酸外切酶处理鉴定，片段的连锁关系通过单、双酶切实验和交叉杂交推断。基因组的分子量为100 × 10(6)，由一个由重复序列包围的短独特区域组成，重复序列在DNA群体中在两个方向上的数量大致相等，而一个长独特区域只存在于一个方向上。
The genome of an Australian isolate of equine herpesvirus type 1 (equine abortion virus) has been analysed using the restriction endonucleases EcoRI, BglII and BamHI, and a physical map constructed. Terminal fragments were identified by exonuclease treatments, and linkage of fragments was deduced by a combination of single- and double-digest experiments and cross-blot hybridizations. The genome has a mol. wt. of 100 x 10(6) and is comprised of a short unique region bounded by repetitive sequences, which is present in both orientations in approximately equal amounts in the DNA population, and a long unique region existing in only one orientation.