Nested PCR detection of Plasmodium malariae from microscopy confirmed P. falciparum samples in endemic area of NE India
Nested PCR detection of Plasmodium malariae from microscopy confirmed P. falciparum samples in endemic area of NE India
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DOI：
10.14411/fp.2013.041
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发表时间：
2013-11-01
影响因子：
1.6
通讯作者：
Veer, Vijay
中科院分区：
文献类型：
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作者：
Dhiman, Sunil;Goswami, Diganta;Veer, Vijay
本研究评价了OptiMAL-IT (R)法和巢式PCR法检测疟疾寄生虫的性能。利用显微镜和OptiMAL-IT (R)检测在现场对从两个疟疾流行地区随机抽取的76份血样进行疟疾寄生虫检测。PCR检测在实验室使用从FTA （TM）经典卡片上收集的相同样本的血斑中提取的DNA进行。在61个现场确认的疟疾阳性样本中，只有58个（95%）在实验室使用显微镜检测为阳性。与镜检相比，OptiMal-IT (R)的敏感性、特异性、阳性预测值、阴性预测值和假发现率分别为93%、83%、95%、79%和5%。另一方面，PCR检测的敏感性和特异性分别为97%和100%，阳性预测值为100%，阴性预测值为90%，假发现率为0%。OptiMal-IT (R)和PCR检测在疟疾诊断中的总体效能分别为76%和97%。PCR检测结果显示，镜检错误的4份样本和OptiMAL-IT (R)检测的疟原虫特异性抗原（PAN）均感染了Laveran， 1881型疟原虫。除了标准方法之外，这种PCR检测方法还可以从流行病学角度获得每种疟原虫的真实发病率。
The present study evaluates the performance of OptiMAL-IT (R) test and nested PCR assay in detection of malaria parasites. A total of 76 randomly selected blood samples collected from two malaria endemic areas were tested for malaria parasites using microscopy and OptiMAL-IT (R) test in the field. PCR assays were performed in the laboratory using DNA extracted from blood spots of the same samples collected on the FTA (TM) classic cards. Of the total of 61 field confirmed malaria positive samples, only 58 (95%) were detected positive using microscopy in the laboratory. Sensitivity, specificity, positive predictive value, negative predictive value and false discovery rate of OptiMal-IT (R) in comparison to the microscopy were 93%, 83%, 95%, 79% and 5%, respectively. On the other hand, the sensitivity and specificity of PCR assay were 97% and 100 %, respectively, whereas positive predictive value, negative predictive value and false discovery rate were 100%, 90% and 0%, respectively. The overall performance of OptiMal-IT (R) and PCR assays for malaria diagnosis was 76% and 97%, respectively. PCR assay enabled the identification of infection with Plasmodium malariae Laveran, 1881 in four samples misidentified by microscopy and Plasmodium-specific antigen (PAN) identified by the OptiMAL-IT (R) test. In addition to the standard methods, such PCR assay could be useful to obtain the real incidence of each malaria parasite species for epidemiological perspectives.