SENESCENCE AND THE FLUIDITY OF ROSE PETAL MEMBRANES - RELATIONSHIP TO PHOSPHOLIPID-METABOLISM
SENESCENCE AND THE FLUIDITY OF ROSE PETAL MEMBRANES - RELATIONSHIP TO PHOSPHOLIPID-METABOLISM
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DOI：
10.1104/pp.69.2.296
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发表时间：
1982-01-01
期刊：
影响因子：
7.4
通讯作者：
SHINITZKY, M
中科院分区：
文献类型：
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作者：
BOROCHOV, A;HALEVY, AH;SHINITZKY, M
玫瑰花瓣细胞的衰老伴随着膜流动性的逐渐降低，如通过荧光偏振技术测量的。同时，游离甾醇-磷脂比增加。在衰老过程中对整个组织和分离的原生质体进行的实验表明，游离甾醇的水平没有定量变化。磷脂含量下降，但其组成无任何显着变化。测量[32 P]PE掺入的实验结果表明衰老细胞中磷脂合成的能力降低。青年和老年组织显示磷脂酶A和D的活性，前者随着年龄的增长而增加。显然，玫瑰花瓣膜的流动性随着年龄的增长而降低，这是由于合成减少和降解增强导致磷脂含量减少的结果。质膜的变化进行了讨论。
Senescence of rose petal cells is accompanied by a gradual decrease of membrane fluidity, as measured by a fluorescence polarization technique. Concomitantly, an increase in the free sterol-to-phospholipid ratio occurred. Experiments carried out on whole tissue and isolated protoplasts during senescence revealed that there was no quantitative change in the level of free sterols. The content of phospholipids decreased without any significant change in their composition. Results from experiments measuring the incorporation of [32P]PE indicated a reduced capacity for phospholipid synthesis in senescent cells. Young and old tissue showed phospholipase A and D activity, the former increasing with age. Apparently, the fluidity of rose petal membranes decreases with age as a result of a decrease in phospholipid content, brought about by reduced synthesis and enhanced degradation. Changes in the plasmalemma are discussed.