ASYNCHRONOUS RELEASE OF SYNAPTIC VESICLES DETERMINES THE TIME-COURSE OF THE AMPA RECEPTOR-MEDIATED EPSC
ASYNCHRONOUS RELEASE OF SYNAPTIC VESICLES DETERMINES THE TIME-COURSE OF THE AMPA RECEPTOR-MEDIATED EPSC
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DOI：
10.1016/0896-6273(95)90098-5
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发表时间：
1995-11-01
期刊：
影响因子：
16.2
通讯作者：
JAHR, CE
中科院分区：
文献类型：
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作者：
DIAMOND, JS;JAHR, CE
在培养的海马CA1区神经元中研究了突触间递质扩散对AMPA受体EPSC时程的贡献。降低释放概率20倍镉并没有影响平均AMPA受体EPSC的时间过程中，即使受体脱敏被环噻嗪阻断，这表明个别突触有助于独立的AMPA受体介导的EPSC。从诱发的EPSC的平均微型EPSC的反卷积显示，释放概率衰减仅略快于EPSC，这表明AMPA受体EPSC的时间过程主要是由囊泡释放的稳定性决定。进一步的实验表明，环噻嗪，以前认为只影响AMPA受体动力学，也增强突触释放。
The contribution of intersynaptic transmitter diffusion to the AMPA receptor EPSC time course was studied in cultured CA1 hippocampal neurons. Reducing release probability 20-fold with cadmium did not affect the time course of the averaged AMPA receptor EPSC, even when receptor desensitization was blocked by cyclothiazide, suggesting that individual synapses contribute independently to the AMPA receptor-mediated EPSC. Deconvolution of the averaged miniature EPSC from the evoked EPSC showed that release probability decays only slightly faster than the EPSC, suggesting that the AMPA receptor EPSC time course is determined primarily by the asynchrony of vesicle release. Further experiments demonstrated that cyclothiazide, previously thought to affect only AMPA receptor kinetics, also enhances synaptic release.