Evaluation of antioxidant effect of different extracts of Myrtus communis L.
Evaluation of antioxidant effect of different extracts of Myrtus communis L.
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DOI：
10.1080/10715760310001625609
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发表时间：
2004-01-01
影响因子：
3.3
通讯作者：
Franconi, F
中科院分区：
文献类型：
--
作者：
Romani, A;Coinu, R;Franconi, F
氧化应激参与许多疾病的发病机制。然而，目前还没有找到用于治疗的最佳天然抗氧化剂，因此人们在桃金娘叶中寻找多酚抗氧化剂，桃金娘叶是一种在民间医学中被用作抗炎药的植物。采用不同溶剂从桃金娘 (Myrtus communis L.) 叶液液萃取 (LLE) 中制备出富含抗氧化剂的组分。所有桃金娘提取物都富含多酚。特别地，水醇提取物含有没食子酰葡萄糖苷、鞣花单宁、没食子酰奎尼酸和黄酮醇糖苷；乙酸乙酯提取物和 LLE 后的水残留物分别富含黄酮醇苷和可水解单宁（没食子酰葡萄糖苷、鞣花单宁、没食子酰奎尼酸）。还对单一未鉴定化合物进行了定性和定量分析。使用暴露于铜离子的人类低密度脂蛋白来评估桃金娘提取物的抗氧化活性。添加这些提取物并不影响 LDL 的基础氧化，但剂量依赖性地减少了铜离子诱导的氧化。此外，香桃木提取物减少了共轭二烯的形成。三种香桃木提取物的抗氧化作用按以下顺序降低：水醇提取物、乙酸乙酯和 LLE 后的水残留物。当基于纯化合物校正分子量后考虑总酚化合物的总和时，提取物具有以下IC50：0.36、2.27和2.88μM。统计分析显示，LLE 后水醇提取物与乙酸乙酯和水残留物之间存在显着差异。这些结果表明，桃金娘提取物具有有效的抗氧化活性，主要是由于没食子酰衍生物的存在。
Oxidative stress is involved in the pathogenesis of numerous diseases. Nevertheless, no optimal natural antioxidant has been found for therapeutics, therefore polyphenol antioxidants have been looked for in myrtle leaves, a plant that in folk medicine has been used as anti-inflammatory drug. Antioxidant-rich fractions were prepared from myrtle (Myrtus communis L.) leaves liquid-liquid extraction (LLE) with different solvents. All myrtle extracts were very rich in polyphenols. In particular, hydroalcoholic extracts contain galloyl-glucosides, ellagitannins, galloyl-quinic acids and flavonol glycosides; ethylacetate extract and aqueous residues after LLE are enriched in flavonol glycosides and hydrolysable tannins (galloyl-glucosides, ellagitannins, galloyl-quinic acids), respectively. Qualitative and quantitative analysis for the single unidentified compound was also performed. Human LDL exposed to copper ions was used to evaluate the antioxidant activity of the myrtle extracts. Addition of these extracts did not affect the basal oxidation of LDL but dose-dependently decreased the oxidation induced by copper ions. Moreover, the myrtle extracts reduce the formation of conjugated dienes. The antioxidant effect of three myrtle extracts decreased in the following order: hydroalcoholic extracts, ethylacetate and aqueous residues after LLE. The extracts had the following IC50: 0.36, 2.27 and 2.88 muM, when the sum of total phenolic compounds was considered after the correction of molecular weight based on pure compounds. Statistical analysis showed a significant difference among hydroalcoholic extracts vs. the ethylacetate and aqueous residues after LLE. These results suggest that the myrtle extracts have a potent antioxidant activity mainly due to the presence of galloyl derivatives.