3'-Azido-3'-deoxythymidine-induced reduction in the ability of uninfected CD4-expressing cells to participate in syncytium formation.
3'-Azido-3'-deoxythymidine-induced reduction in the ability of uninfected CD4-expressing cells to participate in syncytium formation.
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3-叠氮基-3-脱氧胸苷诱导未感染的 CD4 表达细胞参与合胞体形成的能力降低。
DOI：
10.1073/pnas.89.17.8361
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发表时间：
1992
影响因子：
11.1
通讯作者：
Shannon,WM
中科院分区：
文献类型：
--
作者：
BuckheitJr,RW;Germany-Decker,J;Qualls-Goodwin,K;Bowdon,BJ;Shannon,WM
由未感染的人类 T 细胞和长期感染 1 型人类免疫缺陷病毒的细胞组成的共培养测定系统已用于研究短期测定中合胞体的形成。用 3'-叠氮基-3'-脱氧胸苷 (AZT) 对未感染的表达 CD4 的人 T 细胞系进行连续处理或短期预处理，会降低这些细胞与慢性感染细胞混合时参与合胞体形成的能力。 AZT 对合胞体形成的影响表现为合胞体数量的减少和检测到的合胞体尺寸的减少。 AZT 的这种合胞体减少作用是剂量和时间依赖性的，并且不是由于未感染、处理的细胞的细胞表面上 CD4 抗原表达的调节所致。当 AZT 持续存在时，观察到最大合胞体减少；然而，预处理时间短至 15 分钟会导致合胞体形成显着减少。由于有效合胞体形成不需要逆转录，因此 AZT 对未感染的人类细胞的合胞体减少作用可能代表了 AZT 的抗病毒特性，具有重要的治疗潜力。
A cocultivation assay system consisting of uninfected human T cells and cells chronically infected with human immunodeficiency virus type 1 has been used to investigate syncytium formation in short-term assays. Continuous treatment or short-term pretreatment of uninfected CD4-expressing human T-cell lines with 3'-azido-3'-deoxythymidine (AZT) reduces the ability of these cells to participate in syncytium formation when mixed with chronically infected cells. The effect of AZT on syncytium formation is observed both as a reduction in the number of syncytia and as a reduction in the size of the syncytia that are detected. This syncytium-reducing effect of AZT is dose and time dependent and does not result from a modulation of CD4 antigen expression on the cell surface of uninfected, treated cells. Maximum syncytium reduction is observed with the continuous presence of AZT; however, pretreatment for times as short as 15 min results in a significant reduction in syncytium formation. Since reverse transcription is not required for efficient syncytium formation, the syncytium-reducing effect of AZT on uninfected human cells may represent an antiviral property of AZT with important therapeutic potential.