PROTEIN TYROSINE PHOSPHATASE-ACTIVITY OF AN ESSENTIAL VIRULENCE DETERMINANT IN YERSINIA
PROTEIN TYROSINE PHOSPHATASE-ACTIVITY OF AN ESSENTIAL VIRULENCE DETERMINANT IN YERSINIA
复制标题
DOI：
10.1126/science.2166336
复制
发表时间：
1990-08-03
期刊：
影响因子：
56.9
通讯作者：
DIXON, JE
中科院分区：
文献类型：
--
作者：
GUAN, KL;DIXON, JE
耶尔森氏菌是鼠疫或黑死病的病原菌，这种微生物曾多次杀死世界上相当一部分人口。耶尔森氏菌的一个重要毒力决定因子是蛋白酪氨酸磷酸酶。重组5-千道尔顿耶尔森氏菌磷酸酶具有特异性去除含Tyr而不是含Ser/Thr的磷酸肽和蛋白质中的磷酸盐。定点突变被用来表明，耶尔森氏菌磷酸酶具有催化所需的基本半胱氨酸残基。一个必需的半胱氨酸残基周围的氨基酸是高度保守的，是其他氨基酸的耶尔森氏菌和哺乳动物蛋白酪氨酸磷酸酶，这表明它们使用一个共同的催化机制。
Yersinia is the genus of bacteria that is the causative agent in plague or the black death, and on several occasions this organism has killed a significant portion of the world''s population. An essential virulence determinant of Yersinia was shown to be a protein tyrosine phosphatase. The recombinant 5-kilodalton Yersinia phosphatase had a specificity for removal of phosphate from Tyr-containing as opposed to Ser/Thr-containing phosphopeptides and proteins. Site-directed mutagenesis was used to show that the Yersinia phosphatase possesses an essential Cys residue required for catalysis. Amino acids surrounding an essential Cys residue are highly conserved, as are other amino acids in the Yersinia and mammalian protein tyrosine phosphatases, suggesting that they use a common catalytic mechanism.