Lighting up carbon monoxide in living cells by a readily available and highly sensitive colorimetric and fluorescent probe
Lighting up carbon monoxide in living cells by a readily available and highly sensitive colorimetric and fluorescent probe
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通过现成的高灵敏度比色和荧光探针点亮活细胞中的一氧化碳
DOI：
10.1016/j.snb.2016.09.023
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发表时间：
2017-03-01
影响因子：
8.4
通讯作者：
Feng, Guoqiang
中科院分区：
文献类型：
--
作者：
Feng, Weiyong;Liu, Dandan;Feng, Guoqiang
一氧化碳（CO）是生命系统中重要的气体递质。这种气体递质的生物检测仍处于起步阶段，迫切需要探针。本文报道了一种基于Pd-0介导的Tsuji-Trost反应的新型荧光探针体系，用于在体外和活细胞中快速检测CO。该探针系统是容易获得的，并显示出优异的传感性能CO，包括快速和独特的比色和荧光开启响应，高选择性和灵敏度与低检测限（25 nM）在水溶液中在温和的条件下。此外，在活细胞中的CO的荧光成像可以方便地实现由低剂量的这种探针系统。(C)2016爱思唯尔B. V.保留所有权利。
Carbon monoxide (CO) is an important gasotransmitter in living systems. The biological detection of this gasotransmitter is still in its infancy and urgently needs probes. In this paper, a novel fluorescent probe system, which is based on the Pd-0-mediated Tsuji-Trost reaction, is reported for rapid detection of CO both in in vitro and in living cells. This probe system is readily available and shows excellent sensing properties for CO including rapid and distinct colorimetric and fluorescent turn-on responses, high selectivity and sensitivity with a low detection limit (25 nM) in aqueous solution under mild conditions. In addition, fluorescent imaging of CO in living cells can be conveniently achieved by a low dose of this probe system. (C) 2016 Elsevier B.V. All rights reserved.