Propofol Prevents Renal Ischemia-Reperfusion Injury via Inhibiting the Oxidative Stress Pathways
Propofol Prevents Renal Ischemia-Reperfusion Injury via Inhibiting the Oxidative Stress Pathways
复制标题
异丙酚通过抑制氧化应激途径预防肾缺血再灌注损伤
DOI：
10.1159/000430329
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Yang, Baoxue
中科院分区：
文献类型：
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作者：
Li, Yingjie;Zhong, Dandan;Yang, Baoxue
背景/目的：肾缺血/再灌注损伤(IRI)是肾移植和心脏手术中急性肾功能衰竭和移植肾功能延迟的危险因素。本研究的目的是确定异丙酚是否具有减轻肾脏IRI的作用，并探讨其作用机制。方法：雄性大鼠右肾切除，左肾行IRI。大鼠在缺血前静脉注射异丙酚。观察肾组织形态和肾功能变化。免疫印迹法检测Bax、Bcl2、caspase-3、GRP78、CHOP和caspase-12的表达。结果：异丙酚预处理的IR大鼠肾功能明显好于未处理的IR大鼠，肾小管细胞凋亡明显减少。异丙酚预处理的IR大鼠Bax/Bcl2比值降低，caspase-3裂解减少。异丙酚预处理后IR大鼠GRP78、CHOP和caspase-12蛋白表达水平显著降低。体外细胞模型显示，异丙酚以剂量依赖的方式显著增加缺氧/复氧(H/R)后NRK-52E细胞的活力。异丙酚在体内外对Bax、Bcl2、Caspase-3、GRP78、CHOP表达的调节作用是一致的。结论：实验结果提示异丙酚通过抑制氧化应激来预防肾脏IRI。
Background/Aims: Renal ischemia/reperfusion injury (IRI) is a risk for acute renal failure and delayed graft function in renal transplantation and cardiac surgery. The purpose of this study is to determine whether propofol could attenuate renal IRI and explore related mechanism. Methods: Male rat right kidney was removed, left kidney was subjected to IRI. Propofol was intravenously injected into rats before ischemia. The kidney morphology and renal function were analyzed. The expression of Bax, Bcl-2, caspase-3, cl-caspase-3, GRP78, CHOP and caspase-12 were detected by Western blot analysis. Results: IR rats with propofol pretreatment had better renal function and less tubular apoptosis than untreated IR rats. Propofol pretreated IR rats had lower Bax/Bcl-2 ratio and less cleaved caspase-3. The protein expression levels of GRP78, CHOP and caspase-12 decreased significantly in propofol pretreated IR rats. In vitro cell model showed that propofol significantly increased the viability of NRK-52E cells that were subjected to hypoxia/reoxygenation (H/R) in a dose-dependent manner. The effect of propofol on the expression regulation of Bax, Bcl-2, caspase-3, GRP78, CHOP was consistent in both in vitro and in vivo models. Conclusion: Experimental results suggest that propofol prevents renal IRI via inhibiting oxidative stress.