PURIFICATION AND SOME PROPERTIES OF PROGENITOR TOXINS OF CLOSTRIDIUM-BOTULINUM TYPE-B
PURIFICATION AND SOME PROPERTIES OF PROGENITOR TOXINS OF CLOSTRIDIUM-BOTULINUM TYPE-B
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DOI：
10.1128/iai.10.4.750-756.1974
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发表时间：
1974-01-01
影响因子：
3.1
通讯作者：
SAKAGUCHI, G
中科院分区：
文献类型：
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作者：
KOZAKI, S;SAKAGUCHI, S;SAKAGUCHI, G
采用酸沉淀、萃取、硫酸铵沉淀、核糖核酸酶消化、酸沉淀、鱼精蛋白处理、磺丙基葡聚糖凝胶层析和Sephadex G-200凝胶过滤等步骤对肉毒梭菌B型秋葵株的祖毒素进行纯化。获得了两种不同分子大小的毒素，命名为大（L）和中（M）毒素。L毒素离心均质，但离心不均一。每毫克氮平均致死量为2.5 × 108 ~ 3.0 × 108，沉降常数为16 S。M毒素离心和离心均质。每毫克氮平均致死量为5.5 × 108 ~ 6.0 × 108，沉降常数为12 S。证明了用过的培养物中存在L和M毒素。因此，将这两种毒素称为祖毒素似乎是合理的。两者都由有毒和无毒成分组成。L毒素和M毒素的毒性成分基本相同。L毒素的无毒成分为12 S，具有血凝活性，约为A型晶体毒素的0.5%，M毒素的无毒成分为7S，无血凝活性。它们有抗原相关性，但不完全相同。
Purification of progenitor toxin ofClostridium botulinumtype B strain Okra was undertaken by sequential steps of acid precipitation, extraction, ammonium sulfate precipitation, ribonuclease digestion, acid precipitation, protamine treatment, sulphopropyl-Sephadex chromatography, and Sephadex G-200 gel filtration. Two different molecular-sized toxins, named large (L) and medium (M) toxins, were obtained. L toxin was centrifugally homogeneous but electrophoretically heterogeneous. It contained 2.5 × 108to 3.0 × 108mean lethal doses per mg of nitrogen, and its sedimentation constant was 16S. M toxin was centrifugally and electrophoretically homogeneous. It contained 5.5 × 108to 6.0 × 108mean lethal doses per mg of nitrogen, and its sedimentation constant was 12S. The presence of both L and M toxins in spent culture was demonstrated. It seems justified, therefore, to call both progenitor toxins. Both consisted of toxic and nontoxic components. The toxic components of L and M toxins appeared to be identical with each other. The nontoxic component of L toxin was 12Sand possessed a hemagglutinin activity of about 0.5% that of type A crystalline toxin; that of M toxin was 7Sand possessed no hemagglutinin activity. They were antigenically related but not identical.