Occurrence of L- and D-crustacean hyperglycemic hormone isoforms in the eyestalk X-organ/sinus gland complex during the ontogeny of the crayfish Astacus leptodactylus
Occurrence of L- and D-crustacean hyperglycemic hormone isoforms in the eyestalk X-organ/sinus gland complex during the ontogeny of the crayfish Astacus leptodactylus
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DOI：
10.1369/jhc.4a6292.2004
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发表时间：
2004-09-01
影响因子：
3.2
通讯作者：
Spanings-Pierrot, C
中科院分区：
文献类型：
--
作者：
Serrano, L;Grousset, E;Spanings-Pierrot, C
利用光学显微镜和免疫细胞化学技术研究了淡水甲壳动物细趾海星（Astacusleptodactylus）眼柄结构的个体发育以及X器官/窦腺（XO/SG）复合体中L-CHH和D-Phe（3）-CHH的合成。视神经节在E1 190 μ m时开始分化（EI：眼指数;孵化时接近410 μ m）。在El 270妈妈，三个髓质（externa，interna，和terminalis）和板ganglionaris存在，并组织在成人眼柄。L-CHH定位于神经内分泌细胞的核周体、神经内分泌细胞束和SG中。D-Phe（3）-CHH在XO胞体中，在其道和胚胎的SG中从El 350 mum和在所有后来的研究阶段中可见。两种CHH立体异构体的共定位总是发生在D-Phe（3）-CHH产生细胞中。这些结果表明，CHH对映体的合成始于胚胎期。leptodactylus，并且D-异构体的合成晚于其L-对应物。我们讨论了翻译后异构化作为产生激素多样性的一种方式，以及D-Phe（3）-CHH合成与β调节能力之间的假定关系，发生在Astacus leptodactylus胚胎生命的后期。
We studied the ontogeny of the eyestalk structure and of the L-CHH and D-Phe(3)-CHH synthesis in the X-organ/sinus gland (XO/SG) complex by light microscopy and immunocytochemistry in the freshwater crustacean Astacus leptodactylus. The optic ganglia start to differentiate in embryos at El 190 mum (EI: eye index; close to 410 mum at hatching). At El 270 mum, the three medullae (externa, interna, and terminalis) and the lamina ganglionaris are present and are organized as in the adult eyestalk. The L-CHH was localized in perikarya of neuroendocrine cells, in their tracts, and in SG from the metanauplius stage to the adult. The D-Phe(3)-CHH was visualized in XO perikarya, in their tracts and in SG of embryos from El 350 mum and in all later studied stages. Co-localization of both CHH stereoisomers always occurred in the D-Phe(3)-CHH-producing cells. These results show that the synthesis of CHH enantiomers starts during the embryonic life in A. leptodactylus, and that the D-isomer is synthesized later than its L-counterpart. We discuss the post-translational isomerization as a way to generate hormonal diversity and the putative relation between D-Phe(3)-CHH synthesis and the ability to osmoregulate, occurring late during the embryonic life of Astacus leptodactylus.