Biotransformation of the SDG in defatted flaxseed into END co-cultured by three single bacterial colonies
Biotransformation of the SDG in defatted flaxseed into END co-cultured by three single bacterial colonies
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脱脂亚麻籽中的 SDG 生物转化为三个单菌落共培养的 END
DOI：
10.1016/j.procbio.2013.10.003
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发表时间：
2014-01-01
影响因子：
4.4
通讯作者：
Liu, Shu-Lin
中科院分区：
文献类型：
--
作者：
Zhu, Hong-Yun;Li, Miao-Xin;Liu, Shu-Lin
肠二醇(End)具有雌激素和抗雌激素活性，可预防乳腺癌、前列腺癌和更年期综合征的发展。本实验室前期研究建立了一种从脱脂亚麻籽的底物中生物转化大量产生第二异落叶松脂醇(SECO)的方法。本研究根据16S rRNA基因序列数据，分离到与利莫氏真细菌种(ZL-II)和莱托埃格氏菌(ZL-III)亲缘关系较近的菌株ZL-II和菌株ZL-III。在菌株ZL-(I+II+III)的联合作用下，脱脂亚麻籽可直接产生END，ZL-II具有脱甲基化活性，ZL-III具有脱羟基活性。菌株ZL-(I+II+III)与亚麻籽中的3-羟基-3-甲基戊二酸以低聚形式存在的二异松脂醇二葡萄糖苷(SDG)在ZL-(I+II+III)共培养条件下可有效转化为末端，转化率可达90%以上。该方法不仅对脱脂亚麻籽的药用价值具有重要意义，而且对亚麻籽原料的资源化利用具有重要意义。(C)爱思唯尔有限公司出版的2013年。
Enterodiol (END) possesses the estrogenic and antiestrogenic activities, which could prevent the development of breast cancer and prostate cancer, as well as menopausal syndrome.Previous studies in our laboratory set up a bio-transformation method for largely yielding secoisolariciresinol (SECO) from the substrate of defatted flaxseeds by strain Bacteroides umformis ZL-I. In this research, another two single colonies, designated as strain ZL-II and strain ZL-III, were isolated, which were closely related to Eubacterium limosum species (ZL-II), and Eggerthella lento species (ZL-III) on the basis of 16S rRNA gene sequence data. Under the combining actions of strains ZL-(I+II+III), END could be produced from defatted flaxseed directly, ZL-II was proved to possess the activities of demethylation, while ZL-III had the activities of dehydroxylation. Secoisolariciresinol diglucoside (SDG) existed in the form of oligomeric with 3-hydroxy-3-methyl glutaric acid in flaxseed could be efficiently transformed into END under the co-culture of strains ZL-(I+II+III), with the conversion rate of more than 90%. The method for mass-producing END from defatted flaxseed reported here is meaningful not only for the medicinal values of END but also for the resource utilization of flaxseed materials. (C) 2013 Published by Elsevier Ltd.