Two-Step Maternal-to-Zygotic Transition with Two-Phase Parental Genome Contributions
Two-Step Maternal-to-Zygotic Transition with Two-Phase Parental Genome Contributions
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具有两阶段亲本基因组贡献的两步母体向合子转变
DOI：
10.1016/j.devcel.2019.04.016
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发表时间：
2019
影响因子：
11.8
通讯作者：
Sun Meng xiang
中科院分区：
文献类型：
--
作者：
Zhao Peng;Zhou Xuemei;Shen Kun;Liu Zhenzhen;Cheng Tianhe;Liu Danni;Cheng Yanbing;Peng Xiongbo;Sun Meng xiang
母体向受精卵的转变(MZT)是植物和动物发育的重要转折点。在植物中，MZT的发生时间和父母对合子转录组的贡献仍然不清楚。在这里，通过克服技术限制，我们描述了拟南芥细胞、受精卵和胚胎转录本的多个阶段。利用这些数据集，我们证明了MZT发生在受精卵发育期间，是一个快速母体转录降解和随后大规模新转录的两步相互关联的过程。双亲对合子转录组的贡献是阶段相关的：球形受精卵的转录组以母系为主，而细长的受精卵转录组表现出相同的亲代贡献。我们的结果表明，植物MZT与动物的MZT相似，表现出典型的两步过程，合子的伸长和分裂需要合子基因组的激活，这表明新转录子对于建立合子的极性和促进胚胎发生是必不可少的。
The maternal-to-zygotic transition (MZT) is an essential developmental turning point in both plants and animals. In plants, the timing of MZT and parental contributions to the zygotic transcriptome remain unclear. Here, by overcoming technical limitations, we characterize theArabidopsisegg cell, zygote, and embryo transcriptomes across multiple stages. Using these datasets, we demonstrate that MZT occurs during zygote development and is a two-step interrelated process of rapid maternal transcript degradation followed by large-scalede novotranscription. Parental contributions to the zygotic transcriptome are stage-dependent: the spherical zygote is characterized by a maternally dominated transcriptome, whereas the elongated zygote transcriptome shows equal parental contributions. Our results show that plant MZT is similar to that in animals, showing a typical two-step process, and that zygotic genome activation is required for zygote elongation and division, indicating thatde novotranscripts are essential for the establishment of zygote polarity and embryogenesis promotion.