Semi-polar root exudates in natural grassland communities
Semi-polar root exudates in natural grassland communities
复制标题
DOI：
10.1002/ece3.5043
复制
发表时间：
2019-05-01
影响因子：
2.6
通讯作者：
Scheel, Dierk
中科院分区：
文献类型：
--
作者：
Dietz, Sophie;Herz, Katharina;Scheel, Dierk
在根际，植物暴露于多种不同的生物和非生物因素，它们通过分泌广泛的次生根代谢物来应对这些因素。到目前为止，在野外条件下，根系分泌物的组成在多大程度上具有物种特异性，受土地利用、局部影响和当地邻里的影响尚不清楚。本研究采用植物计和非靶向液相色谱-质谱（LC-MS）联用方法对德国生物多样性探索园内10种常见草地植物根系分泌物进行了分析，其中牧草和牧草各5种。冗余分析和分层聚类结果显示，除了物种特有的代谢物外，所有物种都有大量的半极性代谢物。化学丰富度和分泌物组成表明，草本植物如车前草和草属植物比禾草植物分泌更多的物种特异性代谢物。相反，草类主要受环境条件的影响。在草本植物和草本植物中，植物功能性状对根系渗出模式的影响较小。总的来说，我们的研究结果证明了在野外条件下获得和非靶向分析半极性代谢物的可行性，并允许对天然草地群落植物分泌物进行更深入的了解。
In the rhizosphere, plants are exposed to a multitude of different biotic and abiotic factors, to which they respond by exuding a wide range of secondary root metabolites. So far, it has been unknown to which degree root exudate composition is species-specific and is affected by land use, the local impact and local neighborhood under field conditions. In this study, root exudates of 10 common grassland species were analyzed, each five of forbs and grasses, in the German Biodiversity Exploratories using a combined phytometer and untargeted liquid chromatography-mass spectrometry (LC-MS) approach. Redundancy analysis and hierarchical clustering revealed a large set of semi-polar metabolites common to all species in addition to species-specific metabolites. Chemical richness and exudate composition revealed that forbs, such as Plantago lanceolata and Galium species, exuded more species-specific metabolites than grasses. Grasses instead were primarily affected by environmental conditions. In both forbs and grasses, plant functional traits had only a minor impact on plant root exudation patterns. Overall, our results demonstrate the feasibility of obtaining and untargeted profiling of semi-polar metabolites under field condition and allow a deeper view in the exudation of plants in a natural grassland community.