Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions.
Excess Glucose Impedes the Proliferation of Skeletal Muscle Satellite Cells Under Adherent Culture Conditions.
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高糖抑制贴壁培养条件下骨骼肌卫星细胞的增殖。
DOI：
10.3389/fcell.2021.640399
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发表时间：
2021
影响因子：
5.5
通讯作者：
Manabe Y
中科院分区：
文献类型：
--
作者：
Furuichi Y;Kawabata Y;Aoki M;Mita Y;Fujii NL;Manabe Y
葡萄糖是增殖的哺乳动物细胞消耗的主要能量来源。因此，一般来说，增殖细胞在细胞外环境中具有高葡萄糖含量的偏好。在这里，我们发现，在贴壁培养条件下，高浓度的葡萄糖阻碍了卫星细胞的增殖，卫星细胞是肌肉特异性干细胞。我们发现，卫星细胞的增殖活性在无葡萄糖的DMEM生长培养基（葡萄糖浓度为2mM的低葡萄糖培养基）中比在标准葡萄糖DMEM（葡萄糖浓度为19mM的高葡萄糖培养基）中更高。卫星细胞培养在高葡萄糖培养基中显示储备细胞的人口减少，确定通过染色Pax7的表达，表明葡萄糖浓度影响细胞命运的决定。总之，葡萄糖是决定骨骼肌特异性干细胞的细胞命运的因素。由于卫星细胞的这种独特特征，高血糖症可能会对骨骼肌肌纤维的再生能力产生负面影响，从而促进肌肉减少症。
Glucose is a major energy source consumed by proliferating mammalian cells. Therefore, in general, proliferating cells have the preference of high glucose contents in extracellular environment. Here, we showed that high glucose concentrations impede the proliferation of satellite cells, which are muscle-specific stem cells, under adherent culture conditions. We found that the proliferation activity of satellite cells was higher in glucose-free DMEM growth medium (low-glucose medium with a glucose concentration of 2 mM) than in standard glucose DMEM (high-glucose medium with a glucose concentration of 19 mM). Satellite cells cultured in the high-glucose medium showed a decreased population of reserve cells, identified by staining for Pax7 expression, suggesting that glucose concentration affects cell fate determination. In conclusion, glucose is a factor that decides the cell fate of skeletal muscle-specific stem cells. Due to this unique feature of satellite cells, hyperglycemia may negatively affect the regenerative capability of skeletal muscle myofibers and thus facilitate sarcopenia.
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