Generation of Induced Pluripotent Stem Cells From Porcine Fibroblasts
Generation of Induced Pluripotent Stem Cells From Porcine Fibroblasts
复制标题
从猪成纤维细胞产生诱导多能干细胞
DOI：
10.3724/sp.j.1206.2009.00737
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发表时间：
2010-06-01
影响因子：
0.3
通讯作者：
Zhang Xiao-Rong
中科院分区：
文献类型：
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作者：
Yin Hui-Qun;Cao Hong-Guo;Zhang Xiao-Rong
为了建立含有特定因子融合蛋白的猪诱导多能干细胞（iPS），采用慢病毒转染技术将4种特定因子基因Oct 4、Sox 2、c-Myc和Klf 4导入猪胎儿成纤维细胞。将表达外源性确定因子基因的猪胎儿成纤维细胞进行传代培养，逐渐分离出清晰的细胞克隆。细胞克隆以相似的速率和稳定性生长，表现出正常的核型，并表达碱性磷酸酶、Oct 4、Nanog和SSEA 1。这些细胞在畸胎瘤中可分化为各种组织。结果证实分离的细胞克隆是iPS细胞。这将极大地促进猪iPS细胞诱导方案的进一步完善和深入研究与应用。
In order to establish pig induced pluripotent stem cells (iPS) with defined factor fusion protein, four defined factors genes Oct4, Sox2, c-Myc and Klf4 were delivered into porcine fetal fibroblasts by lentiviral transfection. The porcine fetal fibroblasts expressed exogenous defined factor genes were sub-cultured, and the clear-cut cell clones were gradually isolated. The cell colones grew at similar rates and stability, exhibited normal karyotype, and expressed alkaline phosphatase, Oct4, Nanog and SSEA1. And these cells could differentiate into various kinds of tissue in teratomas. The results confirmed that the isolated cell clones were iPS cells. This would greatly facilitate the further improvement of the induction protocol and in-depth study and application of pig iPS cells.