Immune destruction of larval taenia crassiceps in mice.
Immune destruction of larval taenia crassiceps in mice.
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小鼠粗头带绦虫幼虫的免疫破坏。
DOI：
10.1128/iai.68.5.2393-2401.2000
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发表时间：
2000
影响因子：
3.1
通讯作者：
Kuhn,RE
中科院分区：
文献类型：
--
作者：
Mooney,KA;Spolski,RJ;See,EJ;Kuhn,RE
用粗带绦虫幼虫芽皮下注射BALB/cJ小鼠，30 ~ 60天后腹腔注射感染，观察其免疫杀伤作用。腹腔内（i. p.）其次是被宿主细胞杀死，宿主细胞将幼虫完全包裹在厚鞘中。通过流式细胞术、酶联免疫吸附试验（ELISA）和逆转录PCR（RT-PCR）对腹腔渗出液细胞及其产生的细胞因子进行了表征。与腹膜内初次感染7天的小鼠相比，在杀死幼虫的小鼠的腹腔中未检测到CD 4 +T细胞、CD 8 +T细胞、B1细胞或巨噬细胞的百分比变化。RT-PCR和ELISA均表明细胞因子包括γ干扰素（IFN-γ）、白细胞介素-4（IL-4）、和IL-10，尽管与单独腹膜内感染7天的小鼠相比几乎没有差异。杀死幼虫的小鼠中的血清细胞因子水平显示IFN-γ和IL-4降低，与单独腹膜内感染30至60天的小鼠相比，IL-10增加，并且与单独腹膜内感染7天的小鼠相比，所有细胞因子增加。抑制一氧化氮的产生并没有显着影响的数量或幼虫的活力在腹腔中的小鼠，杀死幼虫在继发感染。
Immune destruction of larvalTaenia crassicepswas examined by first injecting BALB/cJ mice subcutaneously with larval buds and 30 to 60 days later challenging the mice with larvae injected into the peritoneal cavity. The larvae injected intraperitoneally (i.p.) secondarily are killed by host cells that completely encase the larvae in a thick sheath. The peritoneal exudate cells and the cytokines they produced were characterized by flow cytometry, enzyme-linked immunosorbent assays (ELISAs), and reverse transcription PCR (RT-PCR). No changes in percentage of CD4+T cells, CD8+T cells, B1 cells, or macrophages were detected in the peritoneal cavities of mice that were killing larvae compared to mice with a primary 7-day infection i.p. Both RT-PCR and ELISA demonstrated a decrease in cytokines including gamma interferon (IFN-γ), interleukin-4 (IL-4), and IL-10 in mice that were killing the larvae compared to control mice infected for 30 to 60 days i.p. alone, although there was little difference compared to mice infected for 7 days i.p. alone. Serum cytokine levels in mice that were killing the larvae showed a decrease in IFN-γ and IL-4, an increase in IL-10 when compared to mice infected for 30 to 60 days i.p. alone, and increases in all cytokines compared to mice infected for 7 days i.p. alone. Inhibition of nitric oxide production did not significantly affect the number or the viability of larvae in the peritoneal cavity of mice that were killing larvae during secondary infection.