Amplified electrochemical determination of UO22+ based on the cleavage of the DNAzyme and DNA-modified gold nanoparticle network structure
Amplified electrochemical determination of UO22+ based on the cleavage of the DNAzyme and DNA-modified gold nanoparticle network structure
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基于 DNAzyme 和 DNA 修饰的金纳米颗粒网络结构的裂解放大电化学测定 UO22
DOI：
10.1007/s00604-020-04263-1
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发表时间：
2020-05-04
影响因子：
5.7
通讯作者：
Li, Le
中科院分区：
文献类型：
--
作者：
Cao, Chen;Liu, Jinquan;Li, Le
将DNAzyme与DNA修饰的纳米金（DNA-AuNP）网络结构相结合，设计了一种测定水溶液中UO 22+的上级电化学生物传感器。该方法的主要特点包括UO_（22+）诱导DNAzyme的切割和DNA-AuNP网络结构的信号放大。 在UO 22+存在下，DNA-AuNP网络结构上的特定位点可被UO 22+裂解，释放出DNA-AuNP网络结构，电化学响应强度可检测到降低。电化学响应强度与UO 22+浓度有关。电化学响应强度的对数与UO 22+浓度在10~100 pM范围内呈较宽的线性关系，检出限达到8.1 pM（S/N = 3）。该方法已成功地用于水样中UO 22+的测定。用于增强电信号的装配DNA酶网络结构。数值实验表明，电流信号随着UO 22+浓度的增加而减小。结果表明，该传感器可用于水溶液中UO 22+的有效检测。制造用于增强电信号的DNAzyme网络结构。数值实验表明，电流信号随着UO 22+浓度的增加而减小。结果表明，该传感器可用于水溶液中UO 22+的有效检测。
A superior electrochemical biosensor was designed for the determination of UO22+ in aqueous solution by integration of DNAzyme and DNA-modified gold nanoparticle (DNA-AuNP) network structure. Key features of this method include UO22+ inducing the cleavage of the DNAzyme and signal amplification of DNA-AuNP network structure. In the presence of UO22+, the specific sites on DNA-AuNP network structure can be cleaved by UO22+, releasing the DNA-AuNP network structure with detectable reduction of electrochemical response intensity. The electrochemical response intensity is related to the concentration of UO22+. The logarithm of electrochemical response intensity and UO22+ concentration showed a wide linear range of 10~100 pM, and the detection limit reached 8.1 pM (S/N = 3). This method is successfully used for determination of UO22+ in water samples. Graphical abstract Fabricated DNAzyme network structure for enhanced electrical signal. Numerical experiments show that the current signal decreases as the concentration of UO22+ increases. It can be seen that the biosensors could be used to detect UO22+ in aqueous solution effectively. Fabricated DNAzyme network structure for enhanced electrical signal. Numerical experiments show that the current signal decreases as the concentration of UO22+ increases. It can be seen that the biosensors could be used to detect UO22+ in aqueous solution effectively.