Differential cytotoxicity to human lung normal diploid, virus-transformed and carcinoma cells by the antitumor antibiotics, auromomycin and macromomycin, and their non-protein chromophores.
Differential cytotoxicity to human lung normal diploid, virus-transformed and carcinoma cells by the antitumor antibiotics, auromomycin and macromomycin, and their non-protein chromophores.
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抗肿瘤抗生素、金霉素和大霉素及其非蛋白发色团对人肺正常二倍体、病毒转化细胞和癌细胞的不同细胞毒性。
DOI：
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
S. Okamoto
中科院分区：
文献类型：
--
作者：
N. Miwa;S. Mizuno;S. Okamoto
用集落形成法研究了蛋白质类抗肿瘤抗生素金霉素（AUR）和巨噬霉素（MCR）对正常二倍体成纤维细胞（WI 38）、SV 40转化细胞（VA 13）和肺癌细胞（A549）的细胞毒作用。三种细胞系对抗生素的敏感性依次为：WI 38> VA 13> A549。AUR和MCR之间的细胞毒性效应差异相似。不同的细胞毒性效应不依赖于细胞密度的每一个细胞系在时间的抗肿瘤治疗。抗生素的醇提发色团也显示出类似的差异细胞毒性效应，而抗生素的脱辅基蛋白则没有显示出细胞毒性。结论：AUR和MCR对正常细胞、转化细胞和癌细胞的不同细胞毒作用归因于发色团部分，而蛋白质部分不参与这种作用。
With the use of three human lung cultured cell lines: normal diploid fibroblasts (WI38), their SV40-transformants (VA13) and carcinoma cells (A549), whose doubling times were similar, the cytotoxicity of the protein antitumor antibiotics, auromomycin (AUR) and macromomycin (MCR), was studied by colony formation method. The susceptibilities of the three cell lines to these antibiotics were in the order: WI38 less than VA13 less than A549. This differentiality in the cytotoxic effect was similar between AUR and MCR. The differential cytotoxic effect did not depend on cell densities of each cell line at the time of the antibiotic-treatment. Alcohol-extracted chromophores of the antibiotics also showed a similar differential cytotoxic effect, while the apo-proteins of the antibiotics showed no cytotoxicity. It is concluded that the differential cytotoxic effect of AUR and MCR to normal, transformed and carcinoma cells is attributed to the chromophore moiety and the protein moiety is not involved in this effect.
纯化的金霉素发色团导致脱氧核糖核酸链断裂时需要还原剂。
影响因子：
2.9
作者：
Kappen,LS;Napier,MA;Goldberg,IH;Samy,TS
通讯作者：
Samy,TS