Ganoderma extract activates MAP kinases and induces the neuronal differentiation of rat pheochromocytoma PC12 cells
Ganoderma extract activates MAP kinases and induces the neuronal differentiation of rat pheochromocytoma PC12 cells
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DOI：
10.1016/s0014-5793(00)02317-6
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发表时间：
2000-12-15
期刊：
影响因子：
3.5
通讯作者：
Ip, NY
中科院分区：
文献类型：
--
作者：
Cheung, WMW;Hui, WS;Ip, NY
中药的药理和临床应用已有大量文献记载。我们已经使用了体外模型系统，PC 12细胞，以证明灵芝（灵芝）中的神经活性化合物的存在。灵芝提取物可诱导PC 12细胞的神经元分化，并阻止神经生长因子依赖的PC 12神经元凋亡。此外，正如Erk 1、Erk 2和CREB的磷酸化所证明的那样，灵芝的这些作用可能是通过ras/细胞外信号调节激酶（Erk）和cAMP反应元件结合蛋白（CREB）信号通路介导的。因此，我们的数据不仅提供了介导PC 12细胞神经元分化和神经保护的神经活性化合物存在的第一个证据，而且还揭示了参与其作用的潜在信号分子。(C)2000年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
The pharmacology and clinical application of traditional Chinese medicine has been extensively documented. We have used an in vitro model system, PC12 cells, to demonstrate the presence of neuroactive compounds in Ganoderma lucidum (lingzhi). Ganoderma extract induced the neuronal differentiation of PC12 cells and prevented nerve growth factor-dependent PC12 neurons from apoptosis. Moreover, these effects of ganoderma might be mediated via the ras/extracellular signal-regulated kinase (Erk) and cAMP-response element binding protein (CREB) signaling pathways, as demonstrated by the phosphorylation of Erk1, Erk2 and CREB. Thus, our data not only present the first evidence of the presence of neuroactive compounds that mediate the neuronal differentiation and neuroprotection of the PC12 cells, but also reveal the potential signaling molecules involved in its action. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.