ANALYSIS OF NUCLEAR NUMBERS IN INDIVIDUAL MUSCLE-FIBERS DURING DIFFERENTIATION AND GROWTH - SATELLITE CELL MUSCLE FIBER GROWTH UNIT
ANALYSIS OF NUCLEAR NUMBERS IN INDIVIDUAL MUSCLE-FIBERS DURING DIFFERENTIATION AND GROWTH - SATELLITE CELL MUSCLE FIBER GROWTH UNIT
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DOI：
10.1002/jez.1401910305
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发表时间：
1975-01-01
影响因子：
--
通讯作者：
COOPER, GW
中科院分区：
文献类型：
--
作者：
CARDASIS, CA;COOPER, GW
通过对单个完整肌纤维中核群变化的数值分析，研究了在正常分化和生长过程中多核是如何产生的。用胍分离出产前和产后小鼠的腓肠肌纤维（Cardasis和Cooper， '75）并进行检测。在妊娠第19天首次观察到与肌纤维相关的卫星细胞。每条肌纤维（肌肉+卫星细胞核）的核数在这个年龄平均为83个，出生时为157个，到63日龄时继续增加到354个。然而，增长期间的增长率并不是恒定的。在组织学横截面上对卫星细胞和肌核的估计表明，卫星细胞的百分比从出生时的32%下降到成年时的6%。然而，与单个肌纤维相关的卫星细胞数量，根据这些百分比和整个纤维的核计数计算，仅在2至4周龄之间减少。在头两周龄时皮下注射阿糖胞嘧啶。观察到卫星细胞成对，细胞核异常，卫星细胞百分比升高。这一证据以及对整个纤维中核种群的数值分析进一步支持了卫星细胞从出生到成熟导致肌核增加的假设。
A numerical analysis of changes in the populations of nuclei in individual, intact muscle fibers was made to study how multinucleation arises during normal differentiation and growth. Gastrocnemius muscle fibers from pre‐ and post‐natal mice were isolated with guanidine (Cardasis and Cooper, '75) and examined.Satellite cells associated with muscle fibers were first observed at 19 days of gestation. The number of nuclei per muscle fiber (muscle + satellite cell nuclei) averages 83 at this age, 157 at birth and continues to increase to 354 by 63 days of age. However, the rate of increase during growth is not constant. Estimates of satellite cell and muscle nuclei in histological cross sections indicate that there is a decrease in the percentage of satellite cells from 32% at birth to 6% in the adult. However, the numbers of satellite cells associated with individual muscle fibers, calculated from these percentages and the nuclear counts on whole fibers, decreases only between 2 and 4 weeks of age.Cytosine arabinoside was injected subcutaneously during the first two weeks of age. Pairs of satellite cells, abnormal nuclei and elevated percentages of satellite cells were observed. This evidence as well as the numerical analysis of nuclear populations in whole fibers lends further support to the hypothesis that satellite cells account for the increase in muscle nuclei from birth to maturity.