Surfactants selectively ablate enteric neurons of the rat jejunum.
Surfactants selectively ablate enteric neurons of the rat jejunum.
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发表时间：
1983-11
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通讯作者：
D. A. Fox;M. Epstein;P. Bass
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作者：
D. A. Fox;M. Epstein;P. Bass
表面活性剂是一组非特异性的膜扰动物质，可引起神经损伤。将不同浓度的阳离子表面活性剂苯扎氯铵（BAC）和苄索氯铵，阴离子表面活性剂蓖麻油酸钠、二辛基磺基琥珀酸钠和十二烷基硫酸钠以及非离子表面活性剂Triton X-100每隔5分钟施加到大鼠空肠的血清表面，持续0.5小时，然后用盐水冲洗。在表面活性剂应用后30天，取出经处理和未经处理的空肠段并进行组织学检查。测试的所有表面活性剂均显著减少肌间神经丛中神经节细胞的数量。此外，蓖麻油酸钠显着减少粘膜下神经丛神经节细胞的数量。较高浓度的阳离子剂BAC和苄索氯铵引起全身组织损伤，包括平滑肌破坏、淋巴细胞浸润、肠穿孔和死亡。使用BAC作为原型表面活性剂，肽能神经元的分布和肠电活动进行了检查。BAC治疗显着降低的免疫反应性生长抑素，P物质，甲硫氨酸脑啡肽和血管活性肠肽的肌间神经丛。此外，平滑肌的电特性也发生了改变。BAC处理导致不稳定的，明显扭曲的基本电节律和锋电位产生的改变。这些研究表明，在适当浓度的表面活性剂选择性地消融肌间神经元，并改变肽能神经元的分布和肠电参数在大鼠空肠。
Surfactants, a group of nonspecific membrane perturbating substances, can cause nerve damage. Various concentrations of the cationic surfactants benzalkonium chloride (BAC) and benzethonium chloride, the anionic surfactants sodium ricinoleate, dioctyl sodium sulfosuccinate and sodium lauryl sulfate and the nonionic surfactant Triton X-100 were applied to the serosal surface of the rat jejunum every 5 min for 0.5 hr and then rinsed off with saline. Thirty days after surfactant application, the treated and an untreated segment of jejunum were removed and examined histologically. All surfactants which were tested significantly reduced the number of ganglion cells in the myenteric plexus. In addition, sodium ricinoleate significantly reduced the number of ganglion cells in the submucosal plexus. Higher concentrations of the cationic agents BAC and benzethonium chloride caused a generalized tissue damage including disruption of the smooth muscle, lymphocytic infiltration, intestinal perforation and death. Using BAC as a prototype surfactant, peptidergic neuron distribution and gut electrical activity were examined. BAC treatment markedly reduced the immunoreactivity of somatostatin, substance P, met-enkephalin and vasoactive intestinal peptide in the myenteric plexus. In addition, the electric properties of the smooth muscle were altered. BAC treatment resulted in an erratic, markedly distorted basic electric rhythm and an alteration in spike potential generation. These studies demonstrate that surfactants in appropriate concentrations selectively ablate the myenteric neurons and alter peptidergic neuron distribution and gut electrical parameters in the rat jejunum.