REGULATION OF SURVIVAL OF RAT PACHYTENE SPERMATOCYTES BY LACTATE SUPPLY FROM SERTOLI CELLS
REGULATION OF SURVIVAL OF RAT PACHYTENE SPERMATOCYTES BY LACTATE SUPPLY FROM SERTOLI CELLS
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DOI：
10.1530/jrf.0.0650431
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
VANDERMOLEN, HJ
中科院分区：
文献类型：
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作者：
JUTTE, NHPM;JANSEN, R;VANDERMOLEN, HJ
在没有葡萄糖的情况下，粗线期精母细胞和圆形精子细胞在24小时内死亡的曲细精管片段孵育期间，而支持细胞仍然存活。在葡萄糖存在下孵育的曲细精管片段中，生殖细胞存活至少72 h。乳酸而不是葡萄糖是必不可少的[3 H]尿苷掺入和生存的粗线期精母细胞。如果精母细胞在支持细胞存在下孵育，葡萄糖维持[3 H]尿苷掺入生殖细胞。支持细胞在葡萄糖存在下分泌乳酸，FSH刺激乳酸分泌2-4倍。支持细胞提供的乳酸可以调节粗线期精母细胞的活性和存活。
During incubation of fragments of seminiferous tubules in the absence of glucose, pachytene spermatocytes and round spermatids died within 24 h, while Sertoli cells were still viable. The germ cells survived for at least 72 h in seminiferous tubule fragments which were incubated in the presence of glucose. Lactate rather than glucose is essential for [3H]uridine incorporation and survival of isolated pachytene spermatocytes. If the spermatocytes were incubated in the presence of Sertoli cells, glucose maintained the incorporation of [3H]uridinine into the germ cells. Sertoli cells secreted lactate in the presence of glucose and the lactate secretion was stimulated 2-4 fold by FSH. The activity and survival of pachytene spermatocytes in vitro can be regulated by the supply of lactate from Sertoli cells.