Kinetic and equilibrium metal-ion-binding behaviour reflected in a metal-ion-dependent antigenic determinant in bovine prothrombin. Comparison with bovine prothrombin fragment 1.
Kinetic and equilibrium metal-ion-binding behaviour reflected in a metal-ion-dependent antigenic determinant in bovine prothrombin. Comparison with bovine prothrombin fragment 1.
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牛凝血酶原中金属离子依赖性抗原决定簇反映的动力学和平衡金属离子结合行为。
DOI：
10.1042/bj1930411
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Koehler,KA
中科院分区：
文献类型：
--
作者：
Madar,DA;Hall,TJ;Hiskey,RG;Koehler,KA
兔抗（牛凝血酶原片段1）抗体通过使用片段-1亲和色谱法在不存在金属离子的情况下进行分级分离，并显示出在其与牛片段1或凝血酶原的相互作用中存在金属离子的绝对要求。这些抗体被用来评估蛋白质构象变化的速率常数和平衡金属离子结合到分离的牛片段1和完整的凝血酶原。在片段1和凝血酶原中观察到的构象变化率的密切相似性表明，这两种蛋白质中涉及相同的过程，并且凝血酶原的非片段1区域对片段1区域中的该过程基本上没有影响。平衡金属离子结合的研究表明，在片段1和凝血酶原的金属离子结合过程的细节基本上是相同的。我们的结论是，完整的凝血酶原的片段-1域的金属离子结合行为是与分离的片段1相同。
Rabbit anti-(bovine prothrombin fragment 1) antibodies were fractionated by using fragment-1 affinity chromatography in the absence of metal ions, and showed an absolute requirement for the presence of metal ions in their interactions with bovine fragment 1 or prothrombin. These antibodies were employed to evaluate both the rate constants for a protein conformation change and the equilibrium metal-ion binding to isolated bovine fragment 1 and intact prothrombin. The close similarity of the rates obtained for the conformation change in fragment 1 and those observed in prothrombin indicated that the same process is involved in both proteins and that the non-fragment-1 region of the prothrombin has essentially no effect on this process in the fragment-1 region. Equilibrium metal-ion-binding studies indicate that the details of the metal-ion-binding process in fragment 1 and prothrombin are essentially the same. We conclude that the metal-ion-binding behaviour of the fragment-1 domain of intact prothrombin is identical with that of isolated fragment 1.