Universal vaccine carrier. Liposomes that provide T-dependent help to weak antigens.
Universal vaccine carrier. Liposomes that provide T-dependent help to weak antigens.
复制标题
通用疫苗载体。
作者：
N. Garçon;H. Six
本研究描述了一种胸腺依赖性合成疫苗的设计，该疫苗将为B细胞表位提供通用的T细胞表位。在远交系小鼠中评估同时掺入由Th淋巴细胞识别的肽和亲脂性半抗原和对该半抗原的IgG抗体应答的脂质体。DNP-aminocaproyl phosphatidylethanolamine（DNP-CapPE）是一种经过充分表征的T非依赖性半抗原Ag。HA 2肽来源于流感病毒的血凝素蛋白，含有Th和T细胞毒性淋巴细胞识别的氨基酸序列。此外，HA 2在羧基末端附近含有疏水氨基酸序列，允许其掺入脂质体中。免疫结果显示（i）当DNP-CapPE由不含HA 2肽的脂质体携带时，诱导IgM抗体应答，（ii）携带HA 2和DNP-CapPE的脂质体以剂量依赖性方式引发对HA 2和DNP的IgG抗体应答，（iii）脂质体必须在细胞内加工以引发应答，（iv）该系统导致对DNP的记忆应答，和（v）所有IgG亚类都被引发。这些数据表明，含有HA 2肽的脂质体对T-非依赖性Ag表现出T-依赖性载体效应。这些研究结果的意义进行了讨论，结合使用的脂质体模型的特点。
The design of a thymus-dependent synthetic vaccine that will provide a universal T cell epitope for B cell epitopes is described in this study. Simultaneous incorporation into liposomes of both a peptide recognized by Th lymphocytes and a lipophilic hapten and the IgG antibody responses to this hapten were assessed in outbred mice. DNP-aminocaproyl phosphatidylethanolamine (DNP-CapPE) is a well characterized T-independent hapten Ag. HA2 peptide derived from the hemagglutinin protein of influenza virus contains amino acid sequences recognized by Th and T cytotoxic lymphocytes. In addition, HA2 contains a sequence of hydrophobic amino acids near the carboxyl terminus, allowing its incorporation into liposomes. Results of immunization show that (i), when DNP-CapPE is carried by liposomes without the HA2 peptide, an IgM antibody response is induced, (ii) liposomes carrying both HA2 and DNP-CapPE elicit an IgG antibody response to DNP in a dose-dependent fashion for both HA2 and DNP, (iii) the liposomes must be processed intracellularly in order to elicit a response, (iv) the system leads to a memory response for DNP, and (v) all of the IgG subclasses are elicited. These data suggest that liposomes containing the HA2 peptide exhibit a T-dependent carrier effect for a T-independent Ag. The significance of these findings is discussed in conjunction with the characteristics of the liposome model used.