Paenibacillusalba nov., Isolated from Peat Soil
Paenibacillusalba nov., Isolated from Peat Soil
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DOI：
10.1007/s00284-015-0795-9
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发表时间：
2015-03
影响因子：
2.6
通讯作者：
Hyun-Sook. Kim;Sathiyaraj Srinivasan;Sang-Seob Lee
中科院分区：
文献类型：
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作者：
Hyun-Sook. Kim;Sathiyaraj Srinivasan;Sang-Seob Lee
从俄罗斯泥炭土中分离到一株白色细菌，命名为J20- 6 T。根据16 S rRNA基因序列的相似性，菌株J20- 6 T属于类芽孢杆菌属，与耐冷冻类芽孢杆菌YIM 016 T亲缘关系最近（98.2%），溶藻类芽孢杆菌DSM 5050 TT（97.9%）、软骨素类芽孢杆菌DSM 5051 T（97.4%）、波切恩类芽孢杆菌Gsoil 1138 T（96.9%）和果胶类芽孢杆菌RCB-08 T（96.6%）。细胞为革兰氏阳性、能动、兼性需氧、内生孢子形成和杆状。细胞壁含有MK-7作为主要的甲基萘醌和meso-二氨基庚二酸作为诊断性二氨基酸。主要脂肪酸是antiso-C15：0，主要极性脂质是二磷脂酰甘油和磷脂酰乙醇胺。菌株J20- 6 T的DNA G+C含量为49.9mol%。表型、化学分类学和系统发育学数据清楚地表明，菌株J20- 6 T属于类芽孢杆菌属的新成员，其名称为Paenibacillusbasp。十一月，本文提出模式菌株为J20- 6 T（=KEMC 7302- 005 T = JCM 18165 T）。
A white-colored bacterial strain designated J20-6Twas isolated from peat soil collected in Russia. Based on the 16S rRNA gene sequence similarities, the strain J20-6Tbelonged to the genusPaenibacillus, and the closest relatives werePaenibacillus frigoriresistensYIM 016T(98.2 %),Paenibacillus alginolyticusDSM 5050TT(97.9 %),Paenibacillus chondroitinusDSM 5051T(97.4 %),Paenibacillus pocheonensisGsoil 1138T(96.9 %), andPaenibacillus pectinilyticusRCB-08T(96.6 %). Cells are gram-positive, motile, facultative aerobic, endospore forming, and rod shaped. The cell wall contains MK-7 as the predominant menaquinone andmeso-diaminopimelic acid as the diagnostic diamino acid. The major fatty acid is anteiso-C15:0, and the major polar lipids are diphosphatidylglycerol and phosphatidyl-ethanolamine. The DNA G+C content of the strain J20-6Twas 49.9 mol %. The phenotypic, chemotaxonomic, and phylogenetic data clearly suggest that the strain J20-6Tbelongs to the novel member of the genusPaenibacillus, for which the namePaenibacillusalbasp. nov., is proposed. The type strain is J20-6T(=KEMC 7302-005T= JCM 18165T).