Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation
Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation
复制标题
DOI：
10.1084/jem.20011700
复制
发表时间：
2002-06-17
影响因子：
15.3
通讯作者：
Suda, T
中科院分区：
文献类型：
--
作者：
Arai, F;Ohneda, O;Suda, T
胎儿肢体软骨膜由未分化的间充质细胞组成。然而，该区域细胞的多能性和软骨膜在骨髓形成中的作用尚不清楚。在这份报告中。我们用抗活化白细胞粘附分子（ALCAM）的单克隆抗体纯化和鉴定了软骨膜细胞，并研究了软骨膜细胞在造血骨髓形成中的作用。ALCAM在造血细胞、内皮细胞、骨髓基质细胞和间充质干细胞上表达，并介导嗜同性（ALCAM-ALCAM）/嗜异性（ALCAM-CD 6）细胞粘附。免疫组织化学染色显示ALCAM在软骨膜中表达。通过流式细胞仪分离的ALCAM（+）软骨膜细胞显示出间充质干细胞的特征。ALCAM（+）细胞可以分化为成骨细胞、脂肪细胞、软骨细胞和基质细胞，这些细胞可以支持破骨细胞生成、造血和血管生成。此外，向跖骨培养物中添加ALCAM-Fc或CD 6-Fc，抑制了血管对软骨的侵入。我们的发现表明ALCAM（+）软骨膜细胞通过募集破骨细胞和血管参与血管侵袭。这些发现表明软骨膜可能作为干细胞库，并在骨和骨髓的早期发育中发挥重要作用。
Perichondrium in fetal limb is composed of undifferentiated mesenchymal cells. However, the multipotency of cells in this region and the role of perichondrium in bone marrow formation are not well understood. In this report. we purified and characterized perichondrial cells using a monoclonal antibody against activated leukocyte cell adhesion molecule (ALCAM) and investigated the role of perichondrial cells in hematopoietic bone marrow,, formation. ALCAM is expressed on hematopoietic cells, endothelial cells, bone marrow stromal cells, and mesenchymal stem cells and mediates homophilic (ALCAM-ALCAM)/heterophilic (ALCAM-CD6) cell adhesion. Here we show by immunohistochemical staining that ALCAM is expressed in perichondrium. ALCAM(+) perichondrial cells isolated by FACS(R) exhibit the characteristics of mesenchymal stem cells. ALCAM(+) cells can differentiate into osteoblasts, adipocytes, chondrocytes, and stromal cells, which can support osteoclastogenesis, hematopoiesis, an angiogenesis. Furthermore, the addition of ALCAM-Fc or CD6-Fc to the metatarsal culture, the invasion of the blood vessels to a cartilage was inhibited. Our findings indicate that ALCAM(+) perichondrial cells participate in vascular invasion by recruiting osteoclasts and vessels. These findings suggest that perichondrium might serve as a stem cell reservoir and play an important role in the early development of a bone and bone marrow.