Structural and vascular response of normal and obstructed rabbit whole bladders to distension
Structural and vascular response of normal and obstructed rabbit whole bladders to distension
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DOI：
10.1016/s0090-4295(03)00686-1
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发表时间：
2003-12-01
期刊：
影响因子：
2.1
通讯作者：
Levin, RM
中科院分区：
文献类型：
--
作者：
Matsumoto, S;Chichester, P;Levin, RM
目标。目的：探讨兔部分出口梗阻后腹胀的结构和形态学影响。雄性新西兰大白兔30只，随机分为对照组（假手术）和部分出口梗阻组（3周）。每组3只家兔分别膨胀至容量的5%、25%、50%、100%和125%。每个膀胱在缓冲福尔马林中固定体积6至8小时。将膀胱背侧和腹侧的部分封闭，并对其横截面进行评估。定量形态学分析，CD31免疫组化染色。部分出口梗阻导致膀胱重量和容量增加，粘膜、粘膜下层、逼尿肌和浆膜厚度增加。在对照膀胱中，最大的变薄发生在容量的5%至25%之间，而在阻塞组中，最大的变薄发生在容量的25%至50%之间。在不同程度的扩张中，正常膀胱的血管塌陷程度明显高于梗阻膀胱。最后，梗阻膀胱粘膜下血管明显高于正常膀胱。当膀胱膨胀到容量的25%时，正常膀胱的形态发生了明显的变化，而在25%至125%之间变化相对较小。然而，当膀胱扩张到容量的25%至50%时，阻塞膀胱的扩张导致明显的形态学变化，而在50%至125%之间变化相对较小。
Objectives. To investigate the structural and morphologic effect of distension after partial outlet obstruction in rabbits.Methods. Thirty male New Zealand white rabbits were separated into two groups: control (sham) and partial outlet obstruction (3 weeks). Three rabbits from each group were distended to 5%, 25%, 50%, 100%, and 125% of capacity. Each bladder was fixed at the volume in buffered formalin for 6 to 8 hours. Sections of dorsal and ventral bladder were blocked, and cross sections were evaluated. Quantitative morphometry was performed, and CD31 immunohistochemistry was used to characterize the vascularity.Results. Partial outlet obstruction resulted in increased bladder weight and capacity and increased thickness of the mucosa, submucosa, detrusor, and serosa. In the control bladder, the greatest thinning was seen between 5% and 25% capacity, and in the obstructed group, the greatest thinning occurred between 25% and 50%. The level of vascular collapse was significantly greater for the control bladders than for the obstructed bladders at all levels of distension. Finally, the obstructed bladders showed a significantly greater level of vascularity in the submucosa than the control bladders.Conclusions. Normal bladder distension resulted in significant morphologic changes when the bladder was distended to 25% of capacity but changed relatively little between 25% and 125%. However, distension of the obstructed bladder resulted in significant morphologic changes when the bladder was distended from 25% to 50% of capacity but changed relatively little between 50% and 125%.