Change of calretinin expression in the human colon adenocarcinoma cell line HT29 after differentiation
Change of calretinin expression in the human colon adenocarcinoma cell line HT29 after differentiation
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DOI：
10.1016/0167-4889(96)00090-0
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发表时间：
1996-10-11
影响因子：
5.1
通讯作者：
Gotzos, V
中科院分区：
文献类型：
--
作者：
Cargnello, R;Celio, MR;Gotzos, V
钙视网膜蛋白是EF手家族的一种Ca 2+结合蛋白，在结肠腺癌和结肠源性肿瘤细胞系（例如WiDr）中表达，但在正常人类肠细胞中不存在。其功能尚未阐明，但一些证据使我们假设其参与这些细胞的细胞增殖。为了测试钙视网膜蛋白是否与细胞的未分化、增殖或分化状态相关，在人结肠腺癌克隆细胞系HT 29 - 18中研究了其表达，通过用半乳糖替代培养基中的葡萄糖，可以使其分化为肠细胞样细胞（葡萄糖饥饿分化）。用半乳糖处理HT29 - 18细胞导致钙视黄蛋白mRNA水平和蛋白质表达下降，这通过细胞溶质细胞提取物的免疫细胞化学染色和Western印迹分析来证明。这些结果表明，钙视网膜蛋白存在于HT 29 - 18癌细胞中，主要存在于未分化状态的那些细胞中。钙视网膜蛋白参与维持细胞在未分化（癌）状态的可能性进行了讨论。
Calretinin is a Ca2+-binding protein of the EF-hand family which is expressed in colon adenocarcinomas and colon-derived tumor cell lines (e.g. WiDr), but is absent from normal human enterocytes. Its function has not as yet been elucidated, but some lines of evidence lead us to postulate its involvement in cell proliferation in these cells. In order to test whether calretinin is correlated with an undifferentiated, proliferating, or with a differentiated, state of cells, its expression was studied in the human colon adenocarcinoma clonal cell line HT29-18, which can be caused to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (glucose starvation differentiation). Treatment of HT29-18 cells with galactose led to a drop in the calretinin mRNA level and in protein expression as evidenced by immunocytochemical staining and Western blot analysis of cytosolic cell extracts. These results suggest that calretinin is present in HT29-18 cancer cells, mostly in those which are in the undifferentiated state. The possibility that calretinin is involved in maintaining the cells in an undifferentiated (cancerous) state is discussed.