Hair-specific keratins: characterization and expression of a mouse type I keratin gene.
Hair-specific keratins: characterization and expression of a mouse type I keratin gene.
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头发特异性角蛋白：小鼠 I 型角蛋白基因的表征和表达。
DOI：
10.1111/1523-1747.ep12491511
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
G. Vogeli
中科院分区：
文献类型：
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作者：
P. Kaytes;A. McNab;T. Rea;V. Groppi;T. Kawabe;A. Buhl;A. Bertolino;N. Hatzenbuhler;G. Vogeli
为了研究这种角蛋白在毛发生长周期中的调节作用，我们分离并分析了小鼠I型毛发角蛋白家族的一个成员的基因组克隆。编码序列分为7个外显子。基因结构特别是角蛋白和一般的中间丝，因为内含子-外显子边界不位于蛋白质的结构域边界。与羊毛角蛋白基因的比较表明，两个毛发角蛋白基因的剪接位点相对于蛋白质的氨基酸序列位于相同的位置。同样，这些基因的启动子区域的比较显示了几个核苷酸序列保守的区域，包括TATA盒周围的区域和SV40核心增强子序列。此外，在第四内含子中存在高度的序列同源性。原位杂交显示，该基因的转录本首先在小鼠触须角质区相对未分化的近端皮质区域发现。
A genomic clone for a member of the mouse type I hair keratin protein family has been isolated and analyzed in order to study the regulation of this keratin during the hair growth cycle. The coding sequence is divided into seven exons. The gene structure is typical of keratins in particular and intermediate filaments in general in that the intron-exon borders are not located at the domain borders of the protein. Comparison with a sheep wool keratin gene shows that the splice sites in the two hair keratin genes are found at identical locations relative to the amino acid sequence of the proteins. Similarly, comparison of the promoter areas of these genes shows several areas of nucleotide sequence conservation, including the area around the TATA box and an SV40 core enhancer sequence. In addition, a high degree of sequence identity exists in the fourth intron. In situ hybridization shows that transcripts of this gene are first found in the relatively undifferentiated proximal cortex area in the keratogenous zone of mouse vibrissae.