Genetic Diversity and Relationships of 24 Strains of Genus Auricularia (Agaricomycetes) Assessed Using SRAP Markers
Genetic Diversity and Relationships of 24 Strains of Genus Auricularia (Agaricomycetes) Assessed Using SRAP Markers
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使用 SRAP 标记评估 24 种木耳属（伞形孢菌）菌株的遗传多样性和亲缘关系
DOI：
10.1615/intjmedmushrooms.v18.i10.100
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发表时间：
2016-01-01
影响因子：
1.2
通讯作者：
Dai, Yu-Cheng
中科院分区：
文献类型：
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作者：
Du, Ping;Cao, Tian-Xu;Dai, Yu-Cheng
黑木耳属物种因其传统的食用和药用重要性而众所周知。本研究利用序列相关扩增多态性（SRAP）分子标记技术，对来自中国不同生态区的24个艾/缘/艾属菌株的遗传多样性和亲缘关系进行了分析。11对SRAP引物共检测到402个多态性位点，多态性位点率为100%。相似系数在0.662 ~ 0.903之间，平均值为0.73。利用11个引物扩增的SRAP位点数据，采用算术平均值的对群法进行聚类分析，结果表明，24个黑木耳菌株可分为4个类群，相似性为73%。主坐标分析结果与算术平均聚类的对群法一致，第一个信息量最大的坐标占总变异量的74.38%，表明黑木耳属菌株间具有较高的遗传多样性。总之，SRAP分子标记为区分AurieukirIa strams和评价其亲缘关系提供了一个强有力的工具。
Auricularia species are well known for their traditional edible and medicinal importance. In this study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity and relationships of 24 Ai/rim/aria strains obtained from different ecological regions of China. A total of 402 polymorphic loci were detected using 11 SRAP primer combinations with 100% polymorphic loci. The similarity coefficient ranged from 0.662 to 0.903, with an average of 0.73. Cluster analysis using the pair-group method using arithmetic average, based on the data from SRAP loci amplified by 11 primer pans, showed that 24 Auricularia strains were distinguished into 4 groups with 73% similarity. The results of principal coordinate analysis were in accordance with pair-group method using arithmetic average clustering, and the first, most infonnative coordinate accounted for 74.38% of all the variations, which indicated a high level of genetic diversity among Auricularia strains. In general, SRAP markers provide a powerful tool to discriminate AurieukirIa strams and to evaluate then genetic relationships.