Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model.
Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model.
复制标题
基于自体骨髓基质细胞的体外工程软骨修复猪模型骨软骨缺损
DOI：
10.1038/srep40489
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发表时间：
2017-01-13
影响因子：
4.6
通讯作者：
Zhou G
中科院分区：
文献类型：
--
作者：
He A;Liu L;Luo X;Liu Y;Liu Y;Liu F;Wang X;Zhang Z;Zhang W;Liu W;Cao Y;Zhou G
大型骨软骨缺损的功能重建一直是关节外科的一大挑战。已有研究报道了使用骨髓基质细胞(BMSCs)和可生物降解支架修复关节骨软骨缺损的可行性。然而，由于直接细胞-支架结构植入在活体再生方面的不稳定性，在临床移植方面没有取得重大突破。为了克服直接细胞-支架结构植入的缺点，本研究提出了一种体外软骨再生策略，为相对成熟的软骨样组织提供优越的力学性能。我们的策略包括体外软骨工程，骨软骨缺陷的修复，以及活体修复效果的评估。结果表明，BMSC在体外(BEC体外)存在时间依赖性的成熟过程。单独植入BEC体外可以成功地实现软骨和软骨下骨软骨缺损区的组织特异性修复。此外，BEC体外成熟度对修复效果有显著影响。这些结果表明，在体外软骨再生中，使用BMSCs是一种有前景的骨软骨缺损功能重建策略，从而促进了BMSCs软骨再生技术的临床推广。
Functional reconstruction of large osteochondral defects is always a major challenge in articular surgery. Some studies have reported the feasibility of repairing articular osteochondral defects using bone marrow stromal cells (BMSCs) and biodegradable scaffolds. However, no significant breakthroughs have been achieved in clinical translation due to the instability ofin vivocartilage regeneration based on direct cell-scaffold construct implantation. To overcome the disadvantages of direct cell-scaffold construct implantation, the current study proposed anin vitrocartilage regeneration strategy, providing relatively mature cartilage-like tissue with superior mechanical properties. Our strategy involvedin vitrocartilage engineering, repair of osteochondral defects, and evaluation ofin vivorepair efficacy. The results demonstrated that BMSC engineered cartilagein vitro(BEC-vitro) presented a time-depended maturation process. The implantation of BEC-vitro alone could successfully realize tissue-specific repair of osteochondral defects with both cartilage and subchondral bone. Furthermore, the maturity level of BEC-vitro had significant influence on the repaired results. These results indicated thatin vitrocartilage regeneration using BMSCs is a promising strategy for functional reconstruction of osteochondral defect, thus promoting the clinical translation of cartilage regeneration techniques incorporating BMSCs.