Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation
Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation
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DOI：
10.1007/s00414-004-0453-4
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发表时间：
2004
影响因子：
2.1
通讯作者：
M. Takamiya;K. Saigusa;R. Kumagai;N. Nakayashiki;Y. Aoki
中科院分区：
文献类型：
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作者：
M. Takamiya;K. Saigusa;R. Kumagai;N. Nakayashiki;Y. Aoki
我们研究了组织型纤溶酶原激活物（tPA）的mRNA表达和炎症细胞动力学的创伤年龄估计在小鼠的瘀伤。从损伤后1小时开始检测中性粒细胞。至8 h，它们在皮下组织和真皮下部积累，此后它们扩展到所有层。损伤后3小时即可检测到巨噬细胞，从144小时起可见中度淋巴细胞浸润。此外，从72 h开始表皮增厚。tPA mRNA表达于伤后1h达高峰，72h略有增加。tPA mRNA在表皮细胞、成纤维细胞和内皮细胞中检测到，在损伤前和损伤后，中性粒细胞从3h开始，巨噬细胞从72h开始。本研究提出了时间依赖性tPA mRNA的表达在创伤愈合过程中的时间组织学特征，这被认为是有用的伤口年龄估计的瘀伤。此外，这表明tPA在组织重塑的第一步中起着重要作用。
We investigated mRNA expression of tissue-type plasminogen activator (tPA) and inflammatory cell dynamics for wound age estimation of bruises in mice. Neutrophils were detected from 1 h post-injury. Up to 8 h, they accumulated in subcutaneous tissue and the lower part of the dermis, and thereafter they extended to all the layers. Macrophages became detectable 3 h post-injury, and moderate infiltration of lymphocytes was seen from 144 h. In addition, epidermal thickening was also seen from 72 h. tPA mRNA expression peaked at 1 h, and increased slightly at 72 h post-injury. tPA mRNA was detected in epidermal cells, fibroblasts, and endothelial cells before and after injury, from 3 h in neutrophils and from 72 h in macrophages, respectively. This study presents the time-dependent expression of tPA mRNA in bruises in relation to temporal histologic characteristics during wound healing, which was considered to be useful for wound age estimation. Furthermore, it is suggested that tPA plays an important role in the first step of tissue remodeling.