Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast
Regulation of Cdc2p and Cdc13p is required for cell cycle arrest induced by defective RNA splicing in fission yeast
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DOI：
10.1074/jbc.m504746200
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发表时间：
2005-09-23
影响因子：
4.8
通讯作者：
Murakami, H
中科院分区：
文献类型：
--
作者：
Shimada, M;Namikawa-Yamada, C;Murakami, H
对裂殖酵母的 cdc 突变体进行筛选，寻找细胞周期停滞与 DNA 损伤检查点无关的突变体，鉴定出 RNA 剪接缺陷的 cdc28 突变体。对 cdc28 细胞中未剪接 RNA 进入有丝分裂的突变体的研究结果鉴定出了 orb5 点突变体。编码酪蛋白激酶 II 催化亚基的 orb5(+) 基因被发现是其他 RNA 代谢缺陷突变体中细胞周期停滞所必需的，但不是 DNA 复制或 DNA 损伤检查点操作所必需的。 wee1(+) 或 rad24(+) 功能的丧失也抑制了几种剪接突变体的停滞。主要 B 型细胞周期蛋白 Cdc13p 的过度表达诱导 cdc28 细胞进入有丝分裂。在剪接缺陷细胞中，Cdc13p 的丰度降低，而 Cdc2p 在酪氨酸 15 上的磷酸化得以维持。这些结果表明，Cdc13p 和 Cdc2p 的调节是剪接突变体中 G(2) 阻滞所必需的。
Screening of cdc mutants of fission yeast for those whose cell cycle arrest is independent of the DNA damage checkpoint identified the RNA splicing- deficient cdc28 mutant. A search for mutants of cdc28 cells that enter mitosis with unspliced RNA resulted in the identification of an orb5 point mutant. The orb5(+) gene, which encodes a catalytic subunit of casein kinase II, was found to be required for cell cycle arrest in other mutants with defective RNA metabolism but not for operation of the DNA replication or DNA damage checkpoints. Loss of function of wee1(+) or rad24(+) also suppressed the arrest of several splicing mutants. Overexpression of the major B- type cyclin Cdc13p induced cdc28 cells to enter mitosis. The abundance of Cdc13p was reduced, and the phosphorylation of Cdc2p on tyrosine 15 was maintained in splicing- defective cells. These results suggest that regulation of Cdc13p and Cdc2p is required for G(2) arrest in splicing mutants.