Passive and facilitated transport in nuclear pore complexes is largely uncoupled
Passive and facilitated transport in nuclear pore complexes is largely uncoupled
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DOI：
10.1074/jbc.m608329200
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发表时间：
2007-02-09
影响因子：
4.8
通讯作者：
Reich, Ziv
中科院分区：
文献类型：
--
作者：
Naim, Bracha;Brumfeld, Vlad;Reich, Ziv
核孔复合体是真核细胞核质间物质交换的唯一通道。它们支持两种转运模式：离子、代谢物和中等大小的大分子的被动扩散，以及蛋白质、RNA和核糖核蛋白复合物的促进性受体介导的易位。一般认为，这两种模式的运输发生通过一个单一的扩散通道位于核孔复合物的中心孔。为了验证这一假设，我们研究了利用相同或不同转运模式的转运分子之间的相互作用。我们发现，这两种运输方式不会相互干扰，但利用特定运输方式的分子确实会阻碍利用相同途径的其他分子的运动。因此，我们的结论是，这两种运输方式在很大程度上是分开的。
Nuclear pore complexes provide the sole gateway for the exchange of material between nucleus and cytoplasm of interphase eukaryotic cells. They support two modes of transport: passive diffusion of ions, metabolites, and intermediate-sized macromolecules and facilitated, receptor-mediated translocation of proteins, RNA, and ribonucleoprotein complexes. It is generally assumed that both modes of transport occur through a single diffusion channel located within the central pore of the nuclear pore complex. To test this hypothesis, we studied the mutual effects between transporting molecules utilizing either the same or different modes of translocation. We find that the two modes of transport do not interfere with each other, but molecules utilizing a particular mode of transport do hinder motion of others utilizing the same pathway. We therefore conclude that the two modes of transport are largely segregated.