cDNA cloning and sequencing of the human ryanodine receptor type 3 (RYR3) reveals a novel alternative splice site in the RYR3 gene
cDNA cloning and sequencing of the human ryanodine receptor type 3 (RYR3) reveals a novel alternative splice site in the RYR3 gene
复制标题
DOI：
10.1016/s0014-5793(98)00124-0
复制
发表时间：
1998-02-27
期刊：
影响因子：
3.5
通讯作者：
Brenig, B
中科院分区：
文献类型：
--
作者：
Leeb, T;Brenig, B
从胎儿脑 cDNA 文库中克隆了人兰尼碱受体 3 型 (RYR3)，并确定了其完整序列（EMBL 登录号 AJ001515）。测序的 cDNA 跨度为 15564 bp，包含 14613 bp 的开放阅读框，相应的蛋白质由 4870 个氨基酸组成，计算分子量为 552 kDa，与兔、水貂和鸡的 RYR3 蛋白的氨基酸序列同一性分别为 96%、95% 和 83%。碱基11569-11650并编码截短的蛋白质。 (C) 1998 年欧洲生化学会联合会。
The human ryanodine receptor type 3 (RYR3) was cloned from a fetal brain cDNA library and its complete sequence was determined (EMBL accession number AJ001515). The sequenced cDNA spanned 15564 bp and contained an open reading frame of 14613 bp, The corresponding protein consisted of 4870 amino acids with a calculated molecular mass of 552 kDa, Amino acid sequence identities to the RYR3 proteins from rabbit, mink, and chicken were 96%, 95%, and 83% respectively, A previously unidentified alternative splice site was detected generating a transcript that lacked bases 11569-11650 and encoded a truncated protein. (C) 1998 Federation of European Biochemical Societies.