Role of illumination intensity in microcystin development using Microcystis aeruginosa as the model algae
Role of illumination intensity in microcystin development using Microcystis aeruginosa as the model algae
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DOI：
10.1007/s11356-017-9888-2
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发表时间：
2017-08
影响因子：
5.8
通讯作者：
Hongbo Liu;Xiao-yan Song;Yongnian Guan;Ding Pan;Yanhua Li;Suyun Xu;Y. Fang
中科院分区：
文献类型：
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作者：
Hongbo Liu;Xiao-yan Song;Yongnian Guan;Ding Pan;Yanhua Li;Suyun Xu;Y. Fang
铜绿微囊藻（M. aeruginosa）是藻华中最常见的蓝藻属之一。在目前的工作中，光照强度对M的生长的影响。对铜绿假单胞菌进行了研究，并开发了一种提取细胞内微囊藻毒素（MC）的研磨方法。研究了MCs培养期间藻类密度、pH、总磷（TP）和总氮（TN）的变化。结果表明，研磨法对MC-YR的提取效率比超声法高275%，而研磨法对MC-RR和MC-LR的提取效率与超声法相近。 M 的最佳照明强度。铜绿假单胞菌的浓度为 19–38 µmol m−2s−1，适宜的 pH 范围为 7.5–10.5。当光照强度 ≤ 38 μmol m−2s−1 时，没有显着观察到细胞外 MC 的主动释放。此外，不同光照强度下的细胞内MC产率被发现处于相对稳定的水平。然而，过量的光照强度（≥ 47 μmol m−2s−1）会导致藻细胞裂解并增加细胞外 MC 的浓度，其中 MC-RR 是主要化合物。计算得出的 MC-RR、MC-LR 和 MC-YR 的细胞内/细胞外 MC 比率分别为 2.38（N=100，SD=2.44）、2.68（N=64，SD=3.48）和 1.25（N=30，SD=1.64）。研究发现强光照强度和细胞裂解是影响细胞外 MC 释放的两个主要因素。
Microcystis aeruginosa(M. aeruginosa) is one of the most common genera of cyanobacteria in algal blooms. In the present work, the impact of the illumination intensity on the growth ofM. aeruginosahas been studied and a grinding method for the extraction of intracellular microcystins (MCs) was developed. The variations of algal density, pH, total phosphorus (TP), and total nitrogen (TN) have been investigated during MCs’ culturing period. Results showed that the extraction efficiency of MC-YR by the grinding method was 275% higher than the sonication method, and the extraction efficiencies of MC-RR and MC-LR by the grinding method were similar to the sonication method. The optimal illumination intensity forM. aeruginosawas found to be 19–38 μmol m−2s−1with suitable pH range of 7.5–10.5. Active release of extracellular MCs was not significantly observed when illumination intensities were ≤ 38 μmol m−2s−1. Furthermore, the intracellular MC yields under different illumination intensities were found to be a relatively stable level. However, excess illumination intensity (≥ 47 μmol m−2s−1) led to the lysis of algal cell and increased the concentrations of extracellular MCs, with MC-RR as the dominant compound. The calculated intracellular/extracellular MCs ratios for MC-RR, MC-LR, and MC-YR were 2.38 (N= 100, SD = 2.44), 2.68 (N= 64, SD = 3.48), and 1.25 (N= 30, SD = 1.64), respectively. Strong illumination intensity and cell lysis were found to be the two major factors influencing the release of extracellular MCs.