[Genetic analysis of a rolled-leaf mutant in rice population of T-DNA insertion].
[Genetic analysis of a rolled-leaf mutant in rice population of T-DNA insertion].
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发表时间：
2003-12
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通讯作者：
G. Shen;Xin Qi Wang;L. Yin;Jiang Wang;Lin Li;Jing Zhang
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作者：
G. Shen;Xin Qi Wang;L. Yin;Jiang Wang;Lin Li;Jing Zhang
在农杆菌介导的转bar基因粳稻中花11的T-DNA插入群体中获得了一个卷叶突变体。通过三代自交，获得一个性状纯化的突变体（R1-A2），并以此为亲本与中花11号杂交。36个F1植株的叶片被卷起并且对除草剂Basta具有抗性。在852株F2代植株中，卷叶与正常叶的分离比例为645：207，符合3：1的分离比例。所有卷叶植株对除草剂Basta具有抗性，所有正常叶植株对除草剂Basta敏感。结果表明，卷叶性状与Basta抗性共分离。利用Ds引物对F2群体中45株卷叶植株和30株正常叶植株的总DNA进行扩增，检测T-DNA的存在。结果表明，卷叶植株均扩增出阳性条带，而正常叶片植株均未扩增出阳性条带。回交亲本R1-A2的F1 B1代植株均为卷叶，以中花11为回交亲本，卷叶与正常叶的分离比例为1：1。综合以上结果，说明卷叶突变体与T-DNA共分离，受单显性基因控制。
A rolled-leaf mutant was obtained in a T-DNA(containing bar gene and Ds element) insertion population, which consist of transgenic japonica rice Zhonghua 11 mediated by Agrobacterium tumefaciens. Through self-hybridization of three generations, one of trait-purified mutants (R1-A2) was obtained and used as parent to cross with variety Zhonghua 11. The leaves of 36 F1 plants investigated were rolled and resistant to herbicide Basta. Among 852 F2 plants, the segregation ratio of rolled leaves to normal leaves(645:207) was consistent with 3:1. All rolled-leaf plants were resistant to herbicide Basta, and all normal leaf plants were sensitive to herbicide Basta. These results showed that the trait of rolled-leaf is co-segregated with Basta resistance. The total DNA of 45 rolled-leaf plants and 30 normal leaf plants in F2 population were amplified to test the presence of T-DNA by Ds primers. The results showed that the positive band were amplified in all rolled-leaf plants, but not in every normal leaf plant. In F1B1 progenies, all plants which derived from backcross parent R1-A2 were rolled leaves; while variety Zhonghua 11 was used as backcross parent, the segregation ratio of rolled-leaf to normal leaf was consistent with 1:1. Taking these data together, it indicated that the rolled-leaf mutant was co-segregation with T-DNA and controlled by single dominant gene.