Chemical Synthesis of K48-Linked Diubiquitin by Incorporation of a Lysine-Linked Auxiliary Handle
Chemical Synthesis of K48-Linked Diubiquitin by Incorporation of a Lysine-Linked Auxiliary Handle
复制标题
通过掺入赖氨酸连接的辅助手柄化学合成 K48 连接的双泛素
DOI：
10.1002/ejoc.201600292
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发表时间：
2016
影响因子：
2.8
通讯作者：
Li Yi-Ming
中科院分区：
文献类型：
--
作者：
Xie Rui-Long;Xu Ling;Li Jia-Bin;Chu Guo-Chao;Wang Tao;Huang Yi-Chao;Li Yi-Ming
We synthesized a lysine‐linked auxiliary‐derived building block, ϵ‐N‐2‐mercapto‐1‐(2,4‐dimethoxyphenyl)ethyl lysine, in 20 % isolated yield over a six‐step route. The new building block is well compatible with Fmoc solid‐phase peptide synthesis (SPPS) and hydrazide‐based native chemical ligation (NCL). Furthermore, we successfully incorporated the new lysine‐linked auxiliary handle into a protein segment and performed the chemical synthesis of K48‐linked diubiquitin with stepwise NCL.
