The Arabidopsis genome sequence as a tool for genome analysis in Brassicaceae.: A comparison of the Arabidopsis and Capsella rubella genomes
The Arabidopsis genome sequence as a tool for genome analysis in Brassicaceae.: A comparison of the Arabidopsis and Capsella rubella genomes
复制标题
DOI：
10.1104/pp.104.040030
复制
发表时间：
2004-06-01
期刊：
影响因子：
7.4
通讯作者：
Schmidt, R
中科院分区：
文献类型：
--
作者：
Boivin, K;Acarkan, A;Schmidt, R
利用注释的拟南芥基因组序列作为工具进行比较分析的拟南芥和荠菜rubella基因组。一组比较随机的，短C。风疹序列与拟南芥中相应序列的比较显示，在序列同一性程度和小插入/缺失的频率方面，比对的蛋白质编码外显子序列不同于比对的内含子或基因间序列。利用拟南芥中的分子标记和表达序列标签对大花荠和苋菜种间杂交群体进行了遗传定位。风疹将所得的八个苋菜连锁群与五条拟南芥染色体的序列图进行比较。共检测到14个共线性片段，它们覆盖了拟南芥染色体序列图的85%和荠菜遗传连锁图的92%。染色体片段的几个融合和分裂以及大的倒位解释了在分析的基因组中观察到的14个共线块的排列。此外，从基因组共线性的小规模偏差的证据被found. Colinearity拟南芥和荠菜基因组之间的共线性比以前报道的拟南芥和不同的芸苔属物种之间的比较更明显。
The annotated Arabidopsis genome sequence was exploited as a tool for carrying out comparative analyses of the Arabidopsis and Capsella rubella genomes. Comparison of a set of random, short C. rubella sequences with the corresponding sequences in Arabidopsis revealed that aligned protein-coding exon sequences differ from aligned intron or intergenic sequences in respect to the degree of sequence identity and the frequency of small insertions/deletions. Molecular-mapped markers and expressed sequence tags derived from Arabidopsis were used for genetic mapping in a population derived from an interspecific cross between Capsella grandiflora and C. rubella. The resulting eight Capsella linkage groups were compared to the sequence maps of the five Arabidopsis chromosomes. Fourteen colinear segments spanning approximately 85% of the Arabidopsis chromosome sequence maps and 92% of the Capsella genetic linkage map were detected. Several fusions and fissions of chromosomal segments as well as large inversions account for the observed arrangement of the 14 colinear blocks in the analyzed genomes. In addition, evidence for small-scale deviations from genome colinearity was found. Colinearity between the Arabidopsis and Capsella genomes is more pronounced than has been previously reported for comparisons between Arabidopsis and different Brassica species.