Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment
Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment
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DOI：
10.1152/ajpcell.00576.2008
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发表时间：
2009-04-01
影响因子：
5.5
通讯作者：
Hwang, Pung-Pung
中科院分区：
文献类型：
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作者：
Horng, Jiun-Lin;Lin, Li-Yih;Hwang, Pung-Pung
洪JL，林LY，黄PP.适应酸性环境的斑马鱼胚胎中富含H+-ATP酶细胞的功能调节。美国生理学杂志细胞生理学296：C682-C692，2009年。首次发表于2009年2月11日; doi：10.1152/ajpcell.00576.2008。-在生物系统中维持内部pH稳态是重要的。在我们以前的研究中，发现富含H+-ATP酶（HR）的细胞负责斑马鱼胚胎发育过程中皮肤中的质子分泌。在这项研究中，斑马鱼胚胎暴露于酸性和碱性沃茨中，以调查在pH值失调时HR细胞酸分泌的调节。我们的研究结果表明，在pH 4的水中，斑马鱼胚胎皮肤上的HR细胞的功能可以上调，不仅通过增加细胞数量，而且通过扩大单个细胞的泌酸功能。我们还确定了一个“肺泡型”的顶端开口的HR细胞的顶端膜的扫描电镜观察下，和肺泡型的顶端开口的密度和大小也增加了pH 4的水。p63和PCNA免疫染色结果还表明，额外的HR细胞在pH 4的水可能分化不仅从离子细胞前体细胞，但也新增殖的上皮干细胞。
Horng JL, Lin LY, Hwang PP. Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment. Am J Physiol Cell Physiol 296: C682-C692, 2009. First published February 11, 2009; doi:10.1152/ajpcell.00576.2008.-It is important to maintain internal pH homeostasis in biological systems. In our previous studies, H+-ATPase-rich (HR) cells were found to be responsible for proton secretion in the skin of zebrafish embryos during development. In this study, zebrafish embryos were exposed to acidic and basic waters to investigate the regulation of HR cell acid secretion during pH disturbances. Our results showed that the function of HR cells on the skin of zebrafish embryos can be upregulated in pH 4 water not only by increasing the cell number but also by enlarging the acid-secreting function of single cells. We also identified an "alveolar-type" apical opening under scanning electron microscopy observations of the apical membrane of HR cells, and the density and size of the alveolar type of apical openings were also increased in pH 4 water. p63 and PCNA immunostaining results also showed that additional HR cells in pH 4 water may be differentiated not only from ionocyte precursor cells but also newly proliferating epithelial stem cells.