Chymotrypsin C (Caldecrin) Is Associated with Enamel Development
Chymotrypsin C (Caldecrin) Is Associated with Enamel Development
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DOI：
10.1177/0022034511418231
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发表时间：
2011-10-01
影响因子：
7.6
通讯作者：
Paine, M. L.
中科院分区：
文献类型：
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作者：
Lacruz, R. S.;Smith, C. E.;Paine, M. L.
两种主要的蛋白酶在釉质形成过程中裂解牙釉质细胞外基质蛋白。基质金属蛋白酶 20 (Mmp20) 是在分泌阶段表达的主要酶，而激肽释放酶相关肽酶 4 (Klk4) 主要在成熟期间表达。 Mmp20 和 Klk4 突变会导致牙釉质表型异常。最近，在对来自牙釉质形成的分泌和成熟阶段的大鼠门牙釉质器官细胞进行的全基因组微阵列分析中，发现丝氨酸蛋白酶胰凝乳蛋白酶 C（caldecrin，Ctrc）在牙釉质成熟过程中显着上调（> 11 倍）。先前的报告表明，Ctrc 表达是胰腺特异性的，尽管在大脑中也发现了低水平的表达。我们在这里报告 Ctrc 在釉质器官中的表达。使用定量 PCR (qPCR) 和蛋白质印迹分析来确认发育中的牙釉质器官中 Ctrc 的表达。 Ctrc 的表达谱与 Klk4 相似，尽管 Ctrc mRNA 水平低于 Klk4，但在成熟阶段相对于分泌阶段显着增加。可能参与牙釉质发育的新丝氨酸蛋白酶的发现对于我们了解调节牙釉质生物矿化的因素具有重要意义。
Two main proteases cleave enamel extracellular matrix proteins during amelogenesis. Matrix metalloprotease-20 (Mmp20) is the predominant enzyme expressed during the secretory stage, while kallikrein-related peptidase-4 (Klk4) is predominantly expressed during maturation. Mutations to both Mmp20 and Klk4 result in abnormal enamel phenotypes. During a recent whole-genome microarray analysis of rat incisor enamel organ cells derived from the secretory and maturation stages of amelogenesis, the serine protease chymotrypsin C (caldecrin, Ctrc) was identified as significantly up-regulated (> 11-fold) during enamel maturation. Prior reports indicate that Ctrc expression is pancreas-specific, albeit low levels were also noted in brain. We here report on the expression of Ctrc in the enamel organ. Quantitative PCR (qPCR) and Western blot analysis were used to confirm the expression of Ctrc in the developing enamel organ. The expression profile of Ctrc is similar to that of Klk4, increasing markedly during the maturation stage relative to the secretory stage, although levels of Ctrc mRNA are lower than for Klk4. The discovery of a new serine protease possibly involved in enamel development has important implications for our understanding of the factors that regulate enamel biomineralization.