A novel mannose-containing sialoprotein adhesin involved in the binding of Candida albicans cells to DMBT1
A novel mannose-containing sialoprotein adhesin involved in the binding of Candida albicans cells to DMBT1
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一种新型含甘露糖唾液蛋白粘附素参与白色念珠菌细胞与 DMBT1 的结合
DOI：
10.1111/omi.12374
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发表时间：
2022
影响因子：
3.7
通讯作者：
Oho T
中科院分区：
文献类型：
--
作者：
Setoguchi D;Nagata E;Oho T
白色念珠菌与致龋性变形链球菌协同作用，使口腔内念珠菌化，引起口腔念珠菌病和幼儿龋齿。C.在口腔组织中的定植。白念珠菌感染是这些传染病发生的重要步骤。唾液凝集素1（DMBT 1），也称为唾液凝集素或gp-340，属于清道夫受体富含半胱氨酸（SRCR）超家族，在先天免疫中具有重要功能。在口腔中，DMBT 1导致微生物粘附于牙釉质和口腔粘膜表面，但C.尚未检测到白念珠菌DMBT 1。在这项研究中，我们研究了C。白念珠菌与DMBT 1特异性结合，与SRCRP 2强结合。N-乙酰神经氨酸和甘露糖以及识别这些糖的凝集素抑制SRCRP 2的结合。分离组分的分子量为25 kDa，含有唾液酸和甘露糖残基，并抑制C。与SRCRP 2结合。25 kDa蛋白在C.通过免疫染色和使用该蛋白质的抗血清的细胞ELISA确认白色念珠菌细胞壁，并且Western印迹显示在念珠菌细胞壁部分中存在25-kDa蛋白质。白色念珠菌这些结果表明，分离的粘附素定位于C.粘附素中唾液酸和甘露糖残基在结合反应中起重要作用。
Candida albicanscolonizes the oral cavity and causes oral candidiasis and early childhood caries synergistically with cariogenicStreptococcus mutans. Colonization of oral tissues withC. albicansis an essential step in the initiation of these infectious diseases. Deleted in malignant brain tumors 1 (DMBT1), also known as salivary agglutinin or gp‐340, belongs to the scavenger receptor cysteine‐rich (SRCR) superfamily and has important functions in innate immunity. In the oral cavity, DMBT1 causes microbial adherence to tooth enamel and oral mucosa surfaces, but the adherence ofC. albicansto DMBT1 has not been examined. In this study, we investigated the binding ofC. albicansto DMBT1 and isolated the fungal components responsible for the binding.Candida albicansspecifically bound to DMBT1 and strongly bound to the peptide domain SRCRP2. Binding to SRCRP2 was inhibited byN‐acetylneuraminic acid and mannose and by lectins recognizing these sugars. The isolated component had a molecular mass of 25 kDa, contained sialic acid and mannose residues, and inhibitedC. albicansbinding to SRCRP2. The localization of the 25‐kDa protein on the surface ofC. albicanscell walls was confirmed by immunostaining and a cell ELISA using an antiserum to the protein, and Western blotting revealed the presence of the 25‐kDa protein in the cell wall fraction ofC. albicans. These results suggest that the isolated adhesin is localized on the surface ofC. albicanscell walls and that sialic acid and mannose residues in the adhesin play a significant role in the binding reaction.